SPIN90 (SH3 Protein Interacting with Nck, 90 kDa), an Adaptor Protein That Is Developmentally Regulated during Cardiac Myocyte Differentiation

Chol Seung Lim, Eui Sun Park, Dae Joong Kim, Young Hwa Song, Soo Hyun Eom, Jang Soo Chun, Jae Hong Kim, Jin Kyu Kim, Dongeun Park, Woo Keun Song

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

In the yeast two-hybrid screening, we have isolated a cDNA clone from a human heart library using Nck Src homology 3 (SH3) domains as bait. The full-length cDNA, which encoded 722 amino acids, was identified as a VIP54-related gene containing an SH3 domain, proline-rich motifs, a serine/threonine-rich region, and a long C-terminal hydrophobic region. We refer to this protein as SPIN90 (SH3 Protein Interacting with Nck, 90 kDa). The amino acid sequence of the SH3 domain has the highest homology with those of Fyn, Yes, and c-Src. SPIN90 was broadly expressed in human tissues; in particular, it was highly expressed in heart, brain, and skeletal muscle, and its expression was developmentally regulated during cardiac myocyte differentiation. SPIN90 is able to bind to the first and third SH3 domains of Nck, in vitro, and is colocalized with Nck at sarcomere Z-discs within cardiac myocytes. Moreover, treatment with antisera raised against SPIN90 disrupted sarcomere structure, suggesting that this protein may play an important role in the maintenance of sarcomere structure and/or in the assembly of myofibrils into sarcomeres.

Original languageEnglish
Pages (from-to)12871-12878
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number16
DOIs
Publication statusPublished - 2001 Apr 20
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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