Spiral reentry waves in confluent layer of HL-1 cardiomyocyte cell lines

Jin Hee Hong, Joon Ho Choi, Tae Yun Kim, Kyoung Jin Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cardiac excitation waves that arise in heart tissues have long been an important research topic because they are related to various cardiac arrhythmia. Investigating their properties based on intact animal whole hearts is important but quite demanding and expensive. Subsequently, dissociated cardiac cell cultures have been used as an alternative. Here, we access the usefulness of cardiomyocyte cell line HL-1 in studying generic properties of cardiac waves. Spontaneous wave activities in confluent populations of HL-1 cells are monitored using a phase-contrast optical mapping system and a microelectrode array recording device. We find that high-density cultures of HL-1 cells can support well-defined reentries. Their conduction velocity and rotation period both increase over few days. The increasing trend of rotation period is opposite to the case of control experiments using primary cultures of mouse atrial cells. The progressive myolysis of HL-1 seems responsible for this difference.

Original languageEnglish
Pages (from-to)1269-1273
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number4
DOIs
Publication statusPublished - 2008 Dec 26

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Reentry
Cardiac Myocytes
Cells
Cell Line
Optical Devices
Microelectrodes
Cell culture
Cardiac Arrhythmias
Animals
Thermodynamic properties
Cell Culture Techniques
Tissue
Equipment and Supplies
Research
Population
Experiments

Keywords

  • Arrhythmia
  • Cardiac reentries
  • HL-1 cardiomyocytes
  • Inter-beat-interval (IBI)
  • Mechanical contraction
  • Mouse atrial cells
  • Multi-electrode array (MEA)
  • Myolysis
  • Myosin
  • Optical mapping
  • Spiral waves

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Spiral reentry waves in confluent layer of HL-1 cardiomyocyte cell lines. / Hong, Jin Hee; Choi, Joon Ho; Kim, Tae Yun; Lee, Kyoung Jin.

In: Biochemical and Biophysical Research Communications, Vol. 377, No. 4, 26.12.2008, p. 1269-1273.

Research output: Contribution to journalArticle

Hong, Jin Hee ; Choi, Joon Ho ; Kim, Tae Yun ; Lee, Kyoung Jin. / Spiral reentry waves in confluent layer of HL-1 cardiomyocyte cell lines. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 377, No. 4. pp. 1269-1273.
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