Split mixotrophy: A novel cultivation strategy to enhance the mixotrophic biomass and lipid yields of Chlorella protothecoides

Sang Jun Sim, Jaemin Joun, Min Eui Hong, Anil Kumar Patel

Research output: Contribution to journalArticle

Abstract

Mixotrophy (M) assumes sum of autotrophic (A) and heterotrophic (H) growths. In this study, a novel split-mixotrophic cultivation strategy (SMCS) developed as better mixotrophy via offering mutual-benefits through gas-exchange at both headspaces while splitting both trophic modes. To quantify synergistic-growth effects in combined-autotrophy and combined-heterotrophy (CA&CH) of SMCS, gross O2-evolution, DIC and DO concentrations were compared with A, H and M. Average 12–14% and 26–32% increase in DIC and DO concentrations were determined respectively in CA and CH than A, H and M. Biomass yield in CA + CH was increased approx.1.5-folds higher than yields of A + H and M regimes. These results show SMCS as better cultivation strategy than the M by increased biomass and lipid yields. Challenges associated with organic carbon can be solved by SMCS viz. chlorophyll loss, organic carbon uptake inhibition. SMCS could be a breakthrough to integrate bacterial process with algae for better bioprocess economy and energy recovery.

Original languageEnglish
Article number121820
JournalBioresource Technology
DOIs
Publication statusPublished - 2019 Nov 1

Fingerprint

mixotrophy
Lipids
Biomass
lipid
biomass
Dacarbazine
Organic carbon
organic carbon
autotrophy
heterotrophy
Chlorophyll
Algae
gas exchange
chlorophyll
Gases
alga
fold
Recovery

Keywords

  • Energy
  • Lipid
  • Microalgae
  • SMCS
  • Split mixotrophy

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Split mixotrophy : A novel cultivation strategy to enhance the mixotrophic biomass and lipid yields of Chlorella protothecoides. / Sim, Sang Jun; Joun, Jaemin; Hong, Min Eui; Patel, Anil Kumar.

In: Bioresource Technology, 01.11.2019.

Research output: Contribution to journalArticle

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