SRC activates TAZ for intestinal tumorigenesis and regeneration

Mi Ran Byun, Jun Ha Hwang, A. Rum Kim, Kyung Min Kim, Jung Il Park, Ho Taek Oh, Eun Sook Hwang, Jeong-Ho Hong

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Proto-oncogene tyrosine-protein kinase Src (cSRC) is involved in colorectal cancer (CRC) development and damage-induced intestinal regeneration, although the cellular mechanisms involved are poorly understood. Here, we report that transcriptional coactivator with PDZ binding domain (TAZ) is activated by cSRC, regulating CRC cell proliferation and tumor formation, where cSRC overexpression increases TAZ expression in CRC cells. In contrast, knockdown of cSRC decreases TAZ expression. Additionally, direct phosphorylation of TAZ at Tyr316 by cSRC stimulates nuclear localization and facilitates transcriptional enhancer factor TEF-3 (TEAD4)-mediated transcription. However, a TAZ phosphorylation mutant significantly decreased cell proliferation, wound healing, colony forming, and tumor formation. In a CRC mouse model, ApcMin/+, activated SRC expression was associated with increased TAZ expression in polyps and TAZ depletion decreased polyp formation. Moreover, intestinal TAZ knockout mice had intestinal regeneration defects following γ-irradiation. Finally, significant correspondence between SRC activation and TAZ overexpression was observed in CRC patients. These results suggest that TAZ is a critical factor for SRC-mediated intestinal tumor formation and regeneration.

Original languageEnglish
Pages (from-to)32-40
Number of pages9
JournalCancer Letters
Volume410
DOIs
Publication statusPublished - 2017 Dec 1

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Keywords

  • APC
  • Colorectal cancer
  • Regeneration
  • SRC
  • TAZ

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Byun, M. R., Hwang, J. H., Kim, A. R., Kim, K. M., Park, J. I., Oh, H. T., Hwang, E. S., & Hong, J-H. (2017). SRC activates TAZ for intestinal tumorigenesis and regeneration. Cancer Letters, 410, 32-40. https://doi.org/10.1016/j.canlet.2017.09.003