ssDNA aptamer-based surface plasmon resonance biosensor for the detection of retinol binding protein 4 for the early diagnosis of type 2 diabetes

Jin Lee Su, Byung Soo Youn, Woo Park Ji, Javed H. Niazi, Seok Kim Yeon, Man Bock Gu

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Retinol binding protein 4 (RBP4) is a useful biomarker in the diagnosis of type 2 diabetes since its level in the serum is higher in insulin-resistant states. Accurate measurement of the serum RBP4 levels is hampered by conventional immunologic methods, such as enzyme-linked immunosorbent assay (ELISA). In this study, therefore, we have developed an aptamer-based surface plasmon resonance (SPR) biosensor that can be used to sense for RBP4 in serum samples. A single-stranded DNA (ssDNA) aptamer that showed high affinity (K d = 0.2 ± 0.03 μM) and specificity to RBP4 was selected. This RBP4-specific aptamer was immobilized on a gold chip and used in a label-free RBP4 detection using SPR. Analysis of RBP4 in artificial serum using SPR was compared with ELISA and Western blot analysis. Our results indicated that the RBP4-specific aptamer-based SPR biosensor gave better dose-dependent responses and was more sensitive than ELISA assays. As such, this RBP4 aptamer-based SPR biosensor can be potentially used to monitor the RBP4 levels within the serum as an indicator of type 2 diabetes.

Original languageEnglish
Pages (from-to)2867-2873
Number of pages7
JournalAnalytical Chemistry
Volume80
Issue number8
DOIs
Publication statusPublished - 2008 Apr 15

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Nucleotide Aptamers
Retinol-Binding Proteins
Single-Stranded DNA
Surface plasmon resonance
Medical problems
Biosensors
Immunosorbents
Assays
Enzymes
Biomarkers
Gold
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ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

ssDNA aptamer-based surface plasmon resonance biosensor for the detection of retinol binding protein 4 for the early diagnosis of type 2 diabetes. / Su, Jin Lee; Youn, Byung Soo; Ji, Woo Park; Niazi, Javed H.; Yeon, Seok Kim; Gu, Man Bock.

In: Analytical Chemistry, Vol. 80, No. 8, 15.04.2008, p. 2867-2873.

Research output: Contribution to journalArticle

Su, Jin Lee ; Youn, Byung Soo ; Ji, Woo Park ; Niazi, Javed H. ; Yeon, Seok Kim ; Gu, Man Bock. / ssDNA aptamer-based surface plasmon resonance biosensor for the detection of retinol binding protein 4 for the early diagnosis of type 2 diabetes. In: Analytical Chemistry. 2008 ; Vol. 80, No. 8. pp. 2867-2873.
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