ssDNA aptamers that recognize diclofenac and 2-anilinophenylacetic acid

Choon Bok Joeng, Javed H. Niazi, Su Jin Lee, Man Bock Gu

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A series of 56 ssDNA aptamer variants that bind to diclofenac (DCF) were selected from an initial pool of 2.4 × 1014 ssDNA molecules by Flu-Mag SELEX process. Sequence analysis of these aptamer variants showed three major groups based on sequence similarity in their random N40 sequences. Out of these, four aptamers designated as D10/DA24, D22, D16, and D3 showed high affinity to DCF with Kd values 100.64, 166.34, 148.73, and 42.7 nM, respectively. Secondary structures of these aptamers showed highly distinct features with typical stem and loop structures. Specificity tests with these four aptamer variants showed that D3 aptamer had higher specificity to DCF followed by 2-anilinophenylacetic acid (2APA), a structural analog of DCF. Whereas aptamers D16 and D22 showed higher specificity to 2APA compared to DCF as target used during selection process. Further, the D10/DA24 aptamer showed high affinity but no specificity to DCF. The DCF aptamers selected can be potential candidates for drug-delivery systems, specific detection of DCF and its derivatives in pharmaceutical preparations and contaminants.

Original languageEnglish
Pages (from-to)5380-5387
Number of pages8
JournalBioorganic and Medicinal Chemistry
Volume17
Issue number15
DOIs
Publication statusPublished - 2009 Aug 1

Keywords

  • Aptamer
  • Diclofenac
  • SELEX
  • ssDNA

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry
  • Biochemistry

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