Stabilin-1 mediates phosphatidylserine-dependent clearance of cell cropses in alternatively activated macrophages

Seung Yoon Park, Mi Yeon Jung, Sung Jin Lee, Kae Bok Kang, Alexei Gratchev, Vladimir Riabov, Julia Kzhyshkowska, In-San Kim

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Stabilin-1 is specifically expressed in alternatively activated macrophages. These macrophages participate in anti-inflammatory and healing processes, and display a high phagocytic capacity. In this study, we provide evidence that stabilin-1 is a membrane receptor that performs a crucial function in the clearance of cell corpses. Stabilin-1 is expressed on the cell surface of alternatively activated macrophages and is recruited to the sites of recognition and engulfment of apoptotic bodies, as well as to early phagosomes. Blocking stabilin-1 in macrophages results in defective engulfment of aged red blood cells. Ectopic expression of stabilin-1 induces the binding and engulfment of aged cells in mouse fibroblast L cells. The binding and phagocytosis are dependent on phosphatidylserine (PS), which is well known as an engulfing ligand. Furthermore, using PS-coated beads, we demonstrate that PS directly interacts with stabilin-1 and is sufficient for stabilin-1-mediated phagocytosis. EGF-like domain repeat in stabilin-1 is responsible for PS recognition and binding. Thus, our results demonstrate that stabilin-1, found on alternatively activated macrophages, is a phagocytic receptor mediating the clearance of apoptotic cells in a PS-dependent manner. Therefore, this protein might play an important role in the maintenance of tissue homeostasis and prevention of autoimmunity.

Original languageEnglish
Pages (from-to)3365-3373
Number of pages9
JournalJournal of Cell Science
Volume122
Issue number18
DOIs
Publication statusPublished - 2009 Sep 15
Externally publishedYes

Fingerprint

Phosphatidylserines
Macrophages
Phagocytosis
Phagosomes
Autoimmunity
Cadaver
Epidermal Growth Factor
Homeostasis
Anti-Inflammatory Agents
Fibroblasts
Erythrocytes
Maintenance
Ligands
Membranes
Proteins

Keywords

  • Aged RBC
  • Apoptotic cells
  • Macrophages
  • Phagocytosis
  • Stabilin-1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Stabilin-1 mediates phosphatidylserine-dependent clearance of cell cropses in alternatively activated macrophages. / Park, Seung Yoon; Jung, Mi Yeon; Lee, Sung Jin; Kang, Kae Bok; Gratchev, Alexei; Riabov, Vladimir; Kzhyshkowska, Julia; Kim, In-San.

In: Journal of Cell Science, Vol. 122, No. 18, 15.09.2009, p. 3365-3373.

Research output: Contribution to journalArticle

Park, SY, Jung, MY, Lee, SJ, Kang, KB, Gratchev, A, Riabov, V, Kzhyshkowska, J & Kim, I-S 2009, 'Stabilin-1 mediates phosphatidylserine-dependent clearance of cell cropses in alternatively activated macrophages', Journal of Cell Science, vol. 122, no. 18, pp. 3365-3373. https://doi.org/10.1242/jcs.049569
Park, Seung Yoon ; Jung, Mi Yeon ; Lee, Sung Jin ; Kang, Kae Bok ; Gratchev, Alexei ; Riabov, Vladimir ; Kzhyshkowska, Julia ; Kim, In-San. / Stabilin-1 mediates phosphatidylserine-dependent clearance of cell cropses in alternatively activated macrophages. In: Journal of Cell Science. 2009 ; Vol. 122, No. 18. pp. 3365-3373.
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