Stabilin-2 mediates homophilic cell-cell interactions via its FAS1 domains

Seung Yoon Park, Mi Yeon Jung, In San Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Stabilin-2 was recently shown to mediate a heterophilic interaction with integrin alphaMbeta2 via its FAS1 domain. Here, we demonstrate that stabilin-2 also mediates homophilic cell-cell interactions. L cells expressing stabilin-2 mediate a significant level of cell aggregation, and this aggregation is significantly inhibited by anti-stabilin-2 antibody. Stabilin-2-mediated aggregation is mediated by homophilic interactions and enhanced in the presence of Ca2+ and Mg2+. Interestingly, exogenous addition of FAS1 domains but not EGF-like domains enhances stabilin-2-mediated cell aggregation, suggesting that exogenous FAS1 domains may form polymeric structure with FAS1 domains of stabilin-2. Together, these data show the participation of stabilin-2 in homophilic cell adhesion and role of FAS1 domains.

Original languageEnglish
Pages (from-to)1375-1380
Number of pages6
JournalFEBS Letters
Volume583
Issue number8
DOIs
Publication statusPublished - 2009 Apr 17
Externally publishedYes

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Keywords

  • Cell-cell interaction
  • FAS1 domain
  • Homophilic
  • Stabilin-2

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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