Stability of DNA, RNA, cytomorphology, and immunoantigenicity in residual thinprep® specimens

Younghye Kim, Kap Ro Choi, Moon Jung Chae, Bong Kyung Shin, Han Kyeom Kim, Aeree Kim, Baek-Hui Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The aim of this study was to evaluate the quality of residual liquid-based preparation (LBP) sample after cytopathologic diagnosis. Cervical swab, body fluid, and thyroid fine-needle aspiration (FNA) samples preserved in ThinPrep PreservCyt® solution were tested. Samples kept frozen at -80 °C and stored at room temperature were tested 12 months after the initial sample collection. Gel electrophotography of GAPDH multiplex PCR, RNA integrity number (RIN) values obtained from Agilent bioanalyzer, cytomorphologic changes, and immunohistochemical staining (cytokeratin, thyroid transcription factor-1 (TTF-1), and D2-40) were used for the evaluation of sample quality. All available samples showed successful amplification products in multiplex PCR. However, RNAs in all residual samples were degraded with low RIN values (RIN < 4). RIN values decreased rapidly when samples were stored at room temperature in LBP medium. Cytomorpholoic evaluation and immunohistochemical staining results revealed no change regardless of storage time or storage temperature. In conclusion, RNAs stored in LBP medium degraded quickly at room temperature. Residual alcohol-based LBP cytologic specimens stored at -80 °C and room temperature showed no change in DNA quality, cytomorphology, and immunoreactivity during at least one year of storage.

Original languageEnglish
Pages (from-to)1064-1072
Number of pages9
JournalAPMIS
Volume121
Issue number11
DOIs
Publication statusPublished - 2013 Nov 1

Fingerprint

RNA Stability
RNA
DNA
Temperature
Multiplex Polymerase Chain Reaction
Staining and Labeling
Body Fluids
Keratins
Fine Needle Biopsy
Thyroid Gland
Gels
Alcohols

Keywords

  • DNA
  • Liquid-based cytology
  • Quality
  • RNA
  • ThinPrep

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Microbiology (medical)
  • Immunology and Allergy

Cite this

Stability of DNA, RNA, cytomorphology, and immunoantigenicity in residual thinprep® specimens. / Kim, Younghye; Choi, Kap Ro; Chae, Moon Jung; Shin, Bong Kyung; Kim, Han Kyeom; Kim, Aeree; Kim, Baek-Hui.

In: APMIS, Vol. 121, No. 11, 01.11.2013, p. 1064-1072.

Research output: Contribution to journalArticle

@article{061a00d9f2a94e5eb01cd3428eede890,
title = "Stability of DNA, RNA, cytomorphology, and immunoantigenicity in residual thinprep{\circledR} specimens",
abstract = "The aim of this study was to evaluate the quality of residual liquid-based preparation (LBP) sample after cytopathologic diagnosis. Cervical swab, body fluid, and thyroid fine-needle aspiration (FNA) samples preserved in ThinPrep PreservCyt{\circledR} solution were tested. Samples kept frozen at -80 °C and stored at room temperature were tested 12 months after the initial sample collection. Gel electrophotography of GAPDH multiplex PCR, RNA integrity number (RIN) values obtained from Agilent bioanalyzer, cytomorphologic changes, and immunohistochemical staining (cytokeratin, thyroid transcription factor-1 (TTF-1), and D2-40) were used for the evaluation of sample quality. All available samples showed successful amplification products in multiplex PCR. However, RNAs in all residual samples were degraded with low RIN values (RIN < 4). RIN values decreased rapidly when samples were stored at room temperature in LBP medium. Cytomorpholoic evaluation and immunohistochemical staining results revealed no change regardless of storage time or storage temperature. In conclusion, RNAs stored in LBP medium degraded quickly at room temperature. Residual alcohol-based LBP cytologic specimens stored at -80 °C and room temperature showed no change in DNA quality, cytomorphology, and immunoreactivity during at least one year of storage.",
keywords = "DNA, Liquid-based cytology, Quality, RNA, ThinPrep",
author = "Younghye Kim and Choi, {Kap Ro} and Chae, {Moon Jung} and Shin, {Bong Kyung} and Kim, {Han Kyeom} and Aeree Kim and Baek-Hui Kim",
year = "2013",
month = "11",
day = "1",
doi = "10.1111/apm.12082",
language = "English",
volume = "121",
pages = "1064--1072",
journal = "Acta pathologica et microbiologica Scandinavica",
issn = "0365-5555",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "11",

}

TY - JOUR

T1 - Stability of DNA, RNA, cytomorphology, and immunoantigenicity in residual thinprep® specimens

AU - Kim, Younghye

AU - Choi, Kap Ro

AU - Chae, Moon Jung

AU - Shin, Bong Kyung

AU - Kim, Han Kyeom

AU - Kim, Aeree

AU - Kim, Baek-Hui

PY - 2013/11/1

Y1 - 2013/11/1

N2 - The aim of this study was to evaluate the quality of residual liquid-based preparation (LBP) sample after cytopathologic diagnosis. Cervical swab, body fluid, and thyroid fine-needle aspiration (FNA) samples preserved in ThinPrep PreservCyt® solution were tested. Samples kept frozen at -80 °C and stored at room temperature were tested 12 months after the initial sample collection. Gel electrophotography of GAPDH multiplex PCR, RNA integrity number (RIN) values obtained from Agilent bioanalyzer, cytomorphologic changes, and immunohistochemical staining (cytokeratin, thyroid transcription factor-1 (TTF-1), and D2-40) were used for the evaluation of sample quality. All available samples showed successful amplification products in multiplex PCR. However, RNAs in all residual samples were degraded with low RIN values (RIN < 4). RIN values decreased rapidly when samples were stored at room temperature in LBP medium. Cytomorpholoic evaluation and immunohistochemical staining results revealed no change regardless of storage time or storage temperature. In conclusion, RNAs stored in LBP medium degraded quickly at room temperature. Residual alcohol-based LBP cytologic specimens stored at -80 °C and room temperature showed no change in DNA quality, cytomorphology, and immunoreactivity during at least one year of storage.

AB - The aim of this study was to evaluate the quality of residual liquid-based preparation (LBP) sample after cytopathologic diagnosis. Cervical swab, body fluid, and thyroid fine-needle aspiration (FNA) samples preserved in ThinPrep PreservCyt® solution were tested. Samples kept frozen at -80 °C and stored at room temperature were tested 12 months after the initial sample collection. Gel electrophotography of GAPDH multiplex PCR, RNA integrity number (RIN) values obtained from Agilent bioanalyzer, cytomorphologic changes, and immunohistochemical staining (cytokeratin, thyroid transcription factor-1 (TTF-1), and D2-40) were used for the evaluation of sample quality. All available samples showed successful amplification products in multiplex PCR. However, RNAs in all residual samples were degraded with low RIN values (RIN < 4). RIN values decreased rapidly when samples were stored at room temperature in LBP medium. Cytomorpholoic evaluation and immunohistochemical staining results revealed no change regardless of storage time or storage temperature. In conclusion, RNAs stored in LBP medium degraded quickly at room temperature. Residual alcohol-based LBP cytologic specimens stored at -80 °C and room temperature showed no change in DNA quality, cytomorphology, and immunoreactivity during at least one year of storage.

KW - DNA

KW - Liquid-based cytology

KW - Quality

KW - RNA

KW - ThinPrep

UR - http://www.scopus.com/inward/record.url?scp=84886244119&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84886244119&partnerID=8YFLogxK

U2 - 10.1111/apm.12082

DO - 10.1111/apm.12082

M3 - Article

VL - 121

SP - 1064

EP - 1072

JO - Acta pathologica et microbiologica Scandinavica

JF - Acta pathologica et microbiologica Scandinavica

SN - 0365-5555

IS - 11

ER -