Stability of DNA, RNA, cytomorphology, and immunoantigenicity in residual thinprep® specimens

Younghye Kim, Kap Ro Choi, Moon Jung Chae, Bong Kyung Shin, Han Kyeom Kim, Aeree Kim, Baek hui Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The aim of this study was to evaluate the quality of residual liquid-based preparation (LBP) sample after cytopathologic diagnosis. Cervical swab, body fluid, and thyroid fine-needle aspiration (FNA) samples preserved in ThinPrep PreservCyt® solution were tested. Samples kept frozen at -80 °C and stored at room temperature were tested 12 months after the initial sample collection. Gel electrophotography of GAPDH multiplex PCR, RNA integrity number (RIN) values obtained from Agilent bioanalyzer, cytomorphologic changes, and immunohistochemical staining (cytokeratin, thyroid transcription factor-1 (TTF-1), and D2-40) were used for the evaluation of sample quality. All available samples showed successful amplification products in multiplex PCR. However, RNAs in all residual samples were degraded with low RIN values (RIN < 4). RIN values decreased rapidly when samples were stored at room temperature in LBP medium. Cytomorpholoic evaluation and immunohistochemical staining results revealed no change regardless of storage time or storage temperature. In conclusion, RNAs stored in LBP medium degraded quickly at room temperature. Residual alcohol-based LBP cytologic specimens stored at -80 °C and room temperature showed no change in DNA quality, cytomorphology, and immunoreactivity during at least one year of storage.

Original languageEnglish
Pages (from-to)1064-1072
Number of pages9
JournalAPMIS
Volume121
Issue number11
DOIs
Publication statusPublished - 2013 Nov

Keywords

  • DNA
  • Liquid-based cytology
  • Quality
  • RNA
  • ThinPrep

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Microbiology (medical)

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