Stabilized magnetic enzyme aggregates on graphene oxide for high performance phenol and bisphenol A removal

Na Liu, Gang Liang, Xinwei Dong, Xiaoli Qi, Jungbae Kim, Yunxian Piao

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We develop a magnetically-separable and stabilized method for the remediation of phenol and bisphenol A in water by the biocatalysis of magnetic enzyme aggregates on graphene oxide (GRO). Both magnetic nanoparticles (MNPs) and tyrosinase (Tyr) were covalently attached on GRO (Mag-CA-Tyr/GRO), and additional glutaraldehyde treatment was performed to construct multi-layers of cross-linked MNPs and Tyr aggregates on GRO (Mag-EC-Tyr/GRO). Mag-EC-Tyr/GRO consisted of thicker layers than the covalent attached method according to atomic force microscopy analysis, and degraded both phenol and bisphenol A in high efficiency. Over broad ranges of temperature and pH, the Mag-EC-Tyr/GRO degraded phenol more efficiently than free and covalent attached Tyr. Mag-EC-Tyr/GRO was more stable than the covalent attached Tyr, and retained over 56% of its initial activity after five cycles of repeated uses for phenol degradation. Owning to the high stability and robustness property, Mag-EC-Tyr/GRO enabled successful degradation of bisphenol A in the environmental water.

Original languageEnglish
Pages (from-to)1026-1034
Number of pages9
JournalChemical Engineering Journal
Volume306
DOIs
Publication statusPublished - 2016 Dec 15

Fingerprint

Graphite
Monophenol Monooxygenase
Phenol
Oxides
Graphene
Phenols
phenol
Enzymes
oxide
enzyme
Nanoparticles
bisphenol A
removal
Degradation
degradation
atomic force microscopy
Water
Remediation
Glutaral
Atomic force microscopy

Keywords

  • Bisphenol A
  • Graphene oxide
  • Magnetic separation
  • Phenol
  • Tyrosinase

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

Stabilized magnetic enzyme aggregates on graphene oxide for high performance phenol and bisphenol A removal. / Liu, Na; Liang, Gang; Dong, Xinwei; Qi, Xiaoli; Kim, Jungbae; Piao, Yunxian.

In: Chemical Engineering Journal, Vol. 306, 15.12.2016, p. 1026-1034.

Research output: Contribution to journalArticle

Liu, Na ; Liang, Gang ; Dong, Xinwei ; Qi, Xiaoli ; Kim, Jungbae ; Piao, Yunxian. / Stabilized magnetic enzyme aggregates on graphene oxide for high performance phenol and bisphenol A removal. In: Chemical Engineering Journal. 2016 ; Vol. 306. pp. 1026-1034.
@article{424346607c534e0db5f7dbab96255874,
title = "Stabilized magnetic enzyme aggregates on graphene oxide for high performance phenol and bisphenol A removal",
abstract = "We develop a magnetically-separable and stabilized method for the remediation of phenol and bisphenol A in water by the biocatalysis of magnetic enzyme aggregates on graphene oxide (GRO). Both magnetic nanoparticles (MNPs) and tyrosinase (Tyr) were covalently attached on GRO (Mag-CA-Tyr/GRO), and additional glutaraldehyde treatment was performed to construct multi-layers of cross-linked MNPs and Tyr aggregates on GRO (Mag-EC-Tyr/GRO). Mag-EC-Tyr/GRO consisted of thicker layers than the covalent attached method according to atomic force microscopy analysis, and degraded both phenol and bisphenol A in high efficiency. Over broad ranges of temperature and pH, the Mag-EC-Tyr/GRO degraded phenol more efficiently than free and covalent attached Tyr. Mag-EC-Tyr/GRO was more stable than the covalent attached Tyr, and retained over 56{\%} of its initial activity after five cycles of repeated uses for phenol degradation. Owning to the high stability and robustness property, Mag-EC-Tyr/GRO enabled successful degradation of bisphenol A in the environmental water.",
keywords = "Bisphenol A, Graphene oxide, Magnetic separation, Phenol, Tyrosinase",
author = "Na Liu and Gang Liang and Xinwei Dong and Xiaoli Qi and Jungbae Kim and Yunxian Piao",
year = "2016",
month = "12",
day = "15",
doi = "10.1016/j.cej.2016.08.012",
language = "English",
volume = "306",
pages = "1026--1034",
journal = "Chemical Engineering Journal",
issn = "1385-8947",
publisher = "Elsevier",

}

TY - JOUR

T1 - Stabilized magnetic enzyme aggregates on graphene oxide for high performance phenol and bisphenol A removal

AU - Liu, Na

AU - Liang, Gang

AU - Dong, Xinwei

AU - Qi, Xiaoli

AU - Kim, Jungbae

AU - Piao, Yunxian

PY - 2016/12/15

Y1 - 2016/12/15

N2 - We develop a magnetically-separable and stabilized method for the remediation of phenol and bisphenol A in water by the biocatalysis of magnetic enzyme aggregates on graphene oxide (GRO). Both magnetic nanoparticles (MNPs) and tyrosinase (Tyr) were covalently attached on GRO (Mag-CA-Tyr/GRO), and additional glutaraldehyde treatment was performed to construct multi-layers of cross-linked MNPs and Tyr aggregates on GRO (Mag-EC-Tyr/GRO). Mag-EC-Tyr/GRO consisted of thicker layers than the covalent attached method according to atomic force microscopy analysis, and degraded both phenol and bisphenol A in high efficiency. Over broad ranges of temperature and pH, the Mag-EC-Tyr/GRO degraded phenol more efficiently than free and covalent attached Tyr. Mag-EC-Tyr/GRO was more stable than the covalent attached Tyr, and retained over 56% of its initial activity after five cycles of repeated uses for phenol degradation. Owning to the high stability and robustness property, Mag-EC-Tyr/GRO enabled successful degradation of bisphenol A in the environmental water.

AB - We develop a magnetically-separable and stabilized method for the remediation of phenol and bisphenol A in water by the biocatalysis of magnetic enzyme aggregates on graphene oxide (GRO). Both magnetic nanoparticles (MNPs) and tyrosinase (Tyr) were covalently attached on GRO (Mag-CA-Tyr/GRO), and additional glutaraldehyde treatment was performed to construct multi-layers of cross-linked MNPs and Tyr aggregates on GRO (Mag-EC-Tyr/GRO). Mag-EC-Tyr/GRO consisted of thicker layers than the covalent attached method according to atomic force microscopy analysis, and degraded both phenol and bisphenol A in high efficiency. Over broad ranges of temperature and pH, the Mag-EC-Tyr/GRO degraded phenol more efficiently than free and covalent attached Tyr. Mag-EC-Tyr/GRO was more stable than the covalent attached Tyr, and retained over 56% of its initial activity after five cycles of repeated uses for phenol degradation. Owning to the high stability and robustness property, Mag-EC-Tyr/GRO enabled successful degradation of bisphenol A in the environmental water.

KW - Bisphenol A

KW - Graphene oxide

KW - Magnetic separation

KW - Phenol

KW - Tyrosinase

UR - http://www.scopus.com/inward/record.url?scp=84982141337&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84982141337&partnerID=8YFLogxK

U2 - 10.1016/j.cej.2016.08.012

DO - 10.1016/j.cej.2016.08.012

M3 - Article

VL - 306

SP - 1026

EP - 1034

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

SN - 1385-8947

ER -