TY - GEN
T1 - STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING
AU - Hur, Jeongsoo
AU - Chung, Aram
N1 - Funding Information:
This work was supported by the Samsung Research Funding and Incubation Center for Future Technology (GrantNo.SRFC-IT1802-03).PrimaryhumanT-cellswerepreparedandhandledwiththeinstitution’sInstitutional ReviewBoard(IRB)approval(#202101091).
Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - A novel microfluidic approach specifically designed to engineer human primary T-lymphocytes effectively with high scalability while maintaining cell functionality is reported. The system employs a unique cell elongation-restoration phenomenon called “cell stretching,” which enables highly effective intracellular delivery of external cargos into cells. Vortical recirculation flows are exerted where cells are hydrodynamically elongated and restored, resulting in effective permeabilization of the cellular membrane. Using the platform, highly efficient (>90%), low-material-cost (<$1), minimally invasive, and high-throughput (106 cells/min) delivery of various cargos into human primary T-cells was achieved, demonstrating the practical utility for chimeric antigen receptor (CAR) T-cell-based cancer immunotherapy.
AB - A novel microfluidic approach specifically designed to engineer human primary T-lymphocytes effectively with high scalability while maintaining cell functionality is reported. The system employs a unique cell elongation-restoration phenomenon called “cell stretching,” which enables highly effective intracellular delivery of external cargos into cells. Vortical recirculation flows are exerted where cells are hydrodynamically elongated and restored, resulting in effective permeabilization of the cellular membrane. Using the platform, highly efficient (>90%), low-material-cost (<$1), minimally invasive, and high-throughput (106 cells/min) delivery of various cargos into human primary T-cells was achieved, demonstrating the practical utility for chimeric antigen receptor (CAR) T-cell-based cancer immunotherapy.
KW - CAR-T Immunotherapy
KW - Gene Delivery
KW - Intracellular Delivery
KW - Microfluidics
KW - T-Cell Engineering
UR - http://www.scopus.com/inward/record.url?scp=85136998962&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136998962
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 515
EP - 516
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -
