STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING

Jeongsoo Hur, Aram Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A novel microfluidic approach specifically designed to engineer human primary T-lymphocytes effectively with high scalability while maintaining cell functionality is reported. The system employs a unique cell elongation-restoration phenomenon called “cell stretching,” which enables highly effective intracellular delivery of external cargos into cells. Vortical recirculation flows are exerted where cells are hydrodynamically elongated and restored, resulting in effective permeabilization of the cellular membrane. Using the platform, highly efficient (>90%), low-material-cost (<$1), minimally invasive, and high-throughput (106 cells/min) delivery of various cargos into human primary T-cells was achieved, demonstrating the practical utility for chimeric antigen receptor (CAR) T-cell-based cancer immunotherapy.

Original languageEnglish
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages515-516
Number of pages2
ISBN (Electronic)9781733419031
Publication statusPublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: 2021 Oct 102021 Oct 14

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period21/10/1021/10/14

Keywords

  • CAR-T Immunotherapy
  • Gene Delivery
  • Intracellular Delivery
  • Microfluidics
  • T-Cell Engineering

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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