Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy

Yongjin Sung, Wonshik Choi, Niyom Lue, Ramachandra R. Dasari, Zahid Yaqoob

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. In addition, we demonstrate chromosomal imaging using a dual-wavelength RTPM, which shows its potential to determine the molecular composition of cellular organelles in live cells.

Original languageEnglish
Article numbere49502
JournalPLoS One
Volume7
Issue number11
DOIs
Publication statusPublished - 2012 Nov 16

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Refractometry
Chromosomes
refractive index
Microscopy
microscopy
Refractive index
Microscopic examination
Coloring Agents
chromosomes
Imaging techniques
image analysis
Organelles
Microscopes
microscopes
Cells
organelles
cells
HT29 Cells
Three-Dimensional Imaging
Molecular Structure

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy. / Sung, Yongjin; Choi, Wonshik; Lue, Niyom; Dasari, Ramachandra R.; Yaqoob, Zahid.

In: PLoS One, Vol. 7, No. 11, e49502, 16.11.2012.

Research output: Contribution to journalArticle

Sung, Yongjin ; Choi, Wonshik ; Lue, Niyom ; Dasari, Ramachandra R. ; Yaqoob, Zahid. / Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy. In: PLoS One. 2012 ; Vol. 7, No. 11.
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