Abstract
Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. In addition, we demonstrate chromosomal imaging using a dual-wavelength RTPM, which shows its potential to determine the molecular composition of cellular organelles in live cells.
| Original language | English |
|---|---|
| Article number | e49502 |
| Journal | PLoS One |
| Volume | 7 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2012 Nov 16 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Medicine(all)
Cite this
Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy. / Sung, Yongjin; Choi, Wonshik; Lue, Niyom; Dasari, Ramachandra R.; Yaqoob, Zahid.
In: PLoS One, Vol. 7, No. 11, e49502, 16.11.2012.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stain-Free Quantification of Chromosomes in Live Cells Using Regularized Tomographic Phase Microscopy
AU - Sung, Yongjin
AU - Choi, Wonshik
AU - Lue, Niyom
AU - Dasari, Ramachandra R.
AU - Yaqoob, Zahid
PY - 2012/11/16
Y1 - 2012/11/16
N2 - Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. In addition, we demonstrate chromosomal imaging using a dual-wavelength RTPM, which shows its potential to determine the molecular composition of cellular organelles in live cells.
AB - Refractive index imaging is a label-free technique that enables long-term monitoring of the internal structures and molecular composition in living cells with minimal perturbation. Existing tomographic methods for the refractive index imaging lack 3-D resolution and result in artifacts that prevent accurate refractive index quantification. To overcome these limitations without compromising the capability to observe a sample in its most native condition, we have developed a regularized tomographic phase microscope (RTPM) enabling accurate refractive index imaging of organelles inside intact cells. With the enhanced accuracy, we quantify the mass of chromosomes in intact living cells, and differentiate two human colon cancer lines, HT-29 and T84 cells, solely based on the non-aqueous (dry) mass of chromosomes. In addition, we demonstrate chromosomal imaging using a dual-wavelength RTPM, which shows its potential to determine the molecular composition of cellular organelles in live cells.
UR - http://www.scopus.com/inward/record.url?scp=84869209197&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84869209197&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0049502
DO - 10.1371/journal.pone.0049502
M3 - Article
C2 - 23166689
AN - SCOPUS:84869209197
VL - 7
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 11
M1 - e49502
ER -