Statistical optimization of medium components for the production of xylanase by Aspergillus niger KK2 in submerged cultivation

Byeong Jo Min, Yang Soon Park, Seong Woo Kang, Yoon Seok Song, Jong Ho Lee, Chulhwan Park, Chan Wha Kim, Seung Wook Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Medium composition was optimized for the production of xylanase by Aspergillus niger KK2 using statistical experimental designs. Corn steep liquor (CSL) and industrial yeast extract (IYE) were the most important factors affecting xylanase activity. The medium that produced the optimum conditions for the production of xylanase contained 3% rice straw, 1% wheat bran, 6.3% CSL, 0.15% IYE, and 0.5% KH2PO4. After 4 days of cultivation under optimized conditions in a 2.5-L stirred tank reactor, the activity and productivity of xylanase were 620 IU/mL and 6,458 IU/L·h, respectively. The highest xylanase activity obtained using the optimized medium was 80% greater than the activity obtained using basal medium. The xylanase activity predicted by a polynomial model was 670 IU/mL.

Original languageEnglish
Pages (from-to)302-307
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Volume12
Issue number3
DOIs
Publication statusPublished - 2007 May 1

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Keywords

  • Aspergillus niger
  • Com steep liquor (CSL)
  • Response surface methodology (RSM)
  • Rice straw
  • Xylanase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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