Staufen1-Mediated mRNA Decay Functions in Adipogenesis

Hana Cho, Kyoung Mi Kim, Sisu Han, Junho Choe, Seung Gu Park, Sun Shim Choi, Yoon Ki Kim

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

The double-stranded RNA binding protein Staufen1 (Stau1) is involved in diverse gene expression pathways. For Stau1-mediated mRNA decay (SMD) in mammals, Stau1 binds to the 3' untranslated region of target mRNA and recruits Upf1 to elicit rapid mRNA degradation. However, the events downstream of Upf1 recruitment and the biological importance of SMD remain unclear. Here we show that SMD involves PNRC2, decapping activity, and 5'-to-3' exonucleolytic activity. In particular, Upf1 serves as an adaptor protein for the association of PNRC2 and Stau1. During adipogenesis, Stau1 and PNRC2 increase in abundance, Upf1 becomes hyperphosphorylated, and consequently SMD efficiency is enhanced. Intriguingly, downregulation of SMD components attenuates adipogenesis in a way that is rescued by downregulation of an antiadipogenic factor, Krüppel-like factor 2 (KLF2), the mRNA of which is identified as a substrate of SMD. Our data thus identify a biological role for SMD in adipogenesis.

Original languageEnglish
Pages (from-to)495-506
Number of pages12
JournalMolecular Cell
Volume46
Issue number4
DOIs
Publication statusPublished - 2012 May 25

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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