Staufen1 regulates diverse classes of mammalian transcripts

Yoon Ki Kim, Luc Furic, Marc Parisien, François Major, Luc DesGroseillers, Lynne E. Maquat

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

It is currently unknown how extensively the double-stranded RNA-binding protein Staufen (Stau)1 is utilized by mammalian cells to regulate gene expression. To date, Stau1 binding to the 3′-untranslated region (3′-UTR) of ADP ribosylation factor (ARF)1 mRNA has been shown to target ARF1 mRNA for Stau1-mediated mRNA decay (SMD). ARF1 SMD depends on translation and recruitment of the nonsense-mediated mRNA decay factor Upf1 to the ARF1 3′-UTR by Stau1. Here, we demonstrate that Stau1 binds to a complex structure within the ARF1 3′-UTR. We also use microarrays to show that 1.1 and 1.0% of the 11 569 HeLa-cell transcripts that were analyzed are upregulated and downregulated, respectively, at least two-fold upon Stau1 depletion in three independently performed experiments. We localize the Stau1 binding site to the 3′-UTR of four mRNAs that we define as natural SMD targets. Additionally, we provide evidence that the efficiency of SMD increases during the differentiation of C2C12 myoblasts to myotubes. We propose that Stau1 influences the expression of a wide variety of physiologic transcripts and metabolic pathways.

Original languageEnglish
Pages (from-to)2670-2681
Number of pages12
JournalEMBO Journal
Volume26
Issue number11
DOIs
Publication statusPublished - 2007 Jun 6

Fingerprint

RNA Stability
3' Untranslated Regions
Messenger RNA
ADP-Ribosylation Factor 1
Nonsense Mediated mRNA Decay
RNA-Binding Proteins
Myoblasts
Skeletal Muscle Fibers
Metabolic Networks and Pathways
HeLa Cells
Down-Regulation
Binding Sites
Gene Expression
Microarrays
Gene expression
Cells

Keywords

  • 3′-UTRs
  • Myogenesis
  • Staufen1 binding sites
  • Staufen1-mediated mRNA decay
  • Upf1

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Kim, Y. K., Furic, L., Parisien, M., Major, F., DesGroseillers, L., & Maquat, L. E. (2007). Staufen1 regulates diverse classes of mammalian transcripts. EMBO Journal, 26(11), 2670-2681. https://doi.org/10.1038/sj.emboj.7601712

Staufen1 regulates diverse classes of mammalian transcripts. / Kim, Yoon Ki; Furic, Luc; Parisien, Marc; Major, François; DesGroseillers, Luc; Maquat, Lynne E.

In: EMBO Journal, Vol. 26, No. 11, 06.06.2007, p. 2670-2681.

Research output: Contribution to journalArticle

Kim, YK, Furic, L, Parisien, M, Major, F, DesGroseillers, L & Maquat, LE 2007, 'Staufen1 regulates diverse classes of mammalian transcripts', EMBO Journal, vol. 26, no. 11, pp. 2670-2681. https://doi.org/10.1038/sj.emboj.7601712
Kim YK, Furic L, Parisien M, Major F, DesGroseillers L, Maquat LE. Staufen1 regulates diverse classes of mammalian transcripts. EMBO Journal. 2007 Jun 6;26(11):2670-2681. https://doi.org/10.1038/sj.emboj.7601712
Kim, Yoon Ki ; Furic, Luc ; Parisien, Marc ; Major, François ; DesGroseillers, Luc ; Maquat, Lynne E. / Staufen1 regulates diverse classes of mammalian transcripts. In: EMBO Journal. 2007 ; Vol. 26, No. 11. pp. 2670-2681.
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