Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines

J. Y. Min, M. H. Park, M. K. Park, K. W. Park, N. W. Lee, T. Kim, H. J. Kim, D. H. Lee

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    Staurosporin, a specific inhibitor of PKC, is widely used in studies of signal transduction pathways. Previous studies have shown that staurosporin induces neurite outgrowth, but the underlying mechanisms remain unclear. Here we report that staurosporin induces neurite outgrowth in HN33 hippocampal cells. Two other PKC inhibitors, Go 6976 (specific for α- and β-isoforms) and rotterlin (a selective inhibitor of PKC δ), have no neuritogenic effect. In addition, staurosporin specifically increases ROS generation. NAC, which inhibits the generation of ROS, suppresses the staurosporin-induced neurite outgrowth in HN33 cells. Further, H2O2 causes neurite outgrowth. Taken together, these results confirm a neuritogenic effect of staurosporin and point to ROS as the signal mediator of staurosporin-induced neurite outgrowth in HN33 hippocampal cells. Theme: Development and regeneration Topic: Neurotrophic factors: receptors and cellular mechanisms

    Original languageEnglish
    Pages (from-to)1821-1826
    Number of pages6
    JournalJournal of Neural Transmission
    Volume113
    Issue number11
    DOIs
    Publication statusPublished - 2006 Nov

    Keywords

    • Neurite outgrowth
    • PKC
    • ROS
    • Staurosporin

    ASJC Scopus subject areas

    • Neurology
    • Clinical Neurology
    • Psychiatry and Mental health
    • Biological Psychiatry

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