Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines

J. Y. Min, M. H. Park, M. K. Park, K. W. Park, N. W. Lee, T. Kim, H. J. Kim, D. H. Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Staurosporin, a specific inhibitor of PKC, is widely used in studies of signal transduction pathways. Previous studies have shown that staurosporin induces neurite outgrowth, but the underlying mechanisms remain unclear. Here we report that staurosporin induces neurite outgrowth in HN33 hippocampal cells. Two other PKC inhibitors, Go 6976 (specific for α- and β-isoforms) and rotterlin (a selective inhibitor of PKC δ), have no neuritogenic effect. In addition, staurosporin specifically increases ROS generation. NAC, which inhibits the generation of ROS, suppresses the staurosporin-induced neurite outgrowth in HN33 cells. Further, H2O2 causes neurite outgrowth. Taken together, these results confirm a neuritogenic effect of staurosporin and point to ROS as the signal mediator of staurosporin-induced neurite outgrowth in HN33 hippocampal cells. Theme: Development and regeneration Topic: Neurotrophic factors: receptors and cellular mechanisms

Original languageEnglish
Pages (from-to)1821-1826
Number of pages6
JournalJournal of Neural Transmission
Volume113
Issue number11
DOIs
Publication statusPublished - 2006 Nov

Keywords

  • Neurite outgrowth
  • PKC
  • ROS
  • Staurosporin

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health
  • Biological Psychiatry

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