Stem Cells Seeded on Multilayered Scaffolds Implanted into an Injured Bladder Rat Model Improves Bladder Function

Kshitiz Raj Shrestha, Seung Hwan Jeon, Ae Ryang Jung, In Gul Kim, Ga Eun Kim, Yong Hyun Park, Soo Hyun Kim, Ji Youl Lee

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Background:: To investigate whether human adipose-derived stem cells (hADSCs) seeded on multilayered poly (l-lactide-co-ɛ-caprolactone) (PLCL) sheets improve bladder function in a rat model of detrusor smooth muscle-removed bladder. Methods:: Male rats were randomly divided into 4 groups: Normal, injury (detrusor smooth muscle-removed bladder), PLCL (detrusor smooth muscle-removed bladder implanted with PLCL sheets), and PLCL + ADSC (detrusor smooth muscle-removed bladder implanted with PLCL sheets seeded with hADSCs). Four weeks after the treatment, physiological, histological, immunohistochemical, and immunoblot analyses were performed. Results:: hADSCs were compatible with PLCL sheets. Further, the physiological study of PLCL + ADSC group showed significant improvement in compliance and contractility suggesting the functional improvement of the bladder. Histological, immunohistochemical and immunoblot analyses revealed the uniform distribution of hADSCs in between PLCL sheets as well as differentiation of hADSCs into smooth muscle cells (SMC) which is illustrated by the expression of SMC markers. Conclusion:: hADSCs seeded on the multilayered PLCL sheets has the potential to differentiate into SMC, thus facilitating the recovery of compliance and contractility of the injured bladder.

Original languageEnglish
Pages (from-to)201-212
Number of pages12
JournalTissue Engineering and Regenerative Medicine
Volume16
Issue number2
DOIs
Publication statusPublished - 2019 Apr 11

Keywords

  • Compliance
  • Smooth muscle
  • Stem cells
  • Tissue engineering
  • Urinary bladder

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Stem Cells Seeded on Multilayered Scaffolds Implanted into an Injured Bladder Rat Model Improves Bladder Function'. Together they form a unique fingerprint.

Cite this