Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

Sang Jun Lee, Laxmi Prasad Thapa, Ju Hun Lee, Han Suk Choi, Sung Bong Kim, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.

Original languageEnglish
Pages (from-to)1904-1910
Number of pages7
JournalProcess Biochemistry
Volume51
Issue number12
DOIs
Publication statusPublished - 2016 Dec 1

Fingerprint

Enterobacter aerogenes
Transcription
Acetates
Up-Regulation
Genes
Glucose
Batch Cell Culture Techniques
Culture Media
2,3-butylene glycol

Keywords

  • 2,3-Butanediol
  • Acetate
  • Enterobacter aerogenes
  • Fermentation
  • Transcriptional regulation

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007. / Lee, Sang Jun; Thapa, Laxmi Prasad; Lee, Ju Hun; Choi, Han Suk; Kim, Sung Bong; Park, Chulhwan; Kim, Seung Wook.

In: Process Biochemistry, Vol. 51, No. 12, 01.12.2016, p. 1904-1910.

Research output: Contribution to journalArticle

Lee, Sang Jun ; Thapa, Laxmi Prasad ; Lee, Ju Hun ; Choi, Han Suk ; Kim, Sung Bong ; Park, Chulhwan ; Kim, Seung Wook. / Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007. In: Process Biochemistry. 2016 ; Vol. 51, No. 12. pp. 1904-1910.
@article{81dc98e1bfac4b9cbf7b008c19cc27bd,
title = "Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007",
abstract = "2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2{\%} with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.",
keywords = "2,3-Butanediol, Acetate, Enterobacter aerogenes, Fermentation, Transcriptional regulation",
author = "Lee, {Sang Jun} and Thapa, {Laxmi Prasad} and Lee, {Ju Hun} and Choi, {Han Suk} and Kim, {Sung Bong} and Chulhwan Park and Kim, {Seung Wook}",
year = "2016",
month = "12",
day = "1",
doi = "10.1016/j.procbio.2016.09.008",
language = "English",
volume = "51",
pages = "1904--1910",
journal = "Process Biochemistry",
issn = "1359-5113",
publisher = "Elsevier BV",
number = "12",

}

TY - JOUR

T1 - Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

AU - Lee, Sang Jun

AU - Thapa, Laxmi Prasad

AU - Lee, Ju Hun

AU - Choi, Han Suk

AU - Kim, Sung Bong

AU - Park, Chulhwan

AU - Kim, Seung Wook

PY - 2016/12/1

Y1 - 2016/12/1

N2 - 2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.

AB - 2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.

KW - 2,3-Butanediol

KW - Acetate

KW - Enterobacter aerogenes

KW - Fermentation

KW - Transcriptional regulation

UR - http://www.scopus.com/inward/record.url?scp=84994504366&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84994504366&partnerID=8YFLogxK

U2 - 10.1016/j.procbio.2016.09.008

DO - 10.1016/j.procbio.2016.09.008

M3 - Article

VL - 51

SP - 1904

EP - 1910

JO - Process Biochemistry

JF - Process Biochemistry

SN - 1359-5113

IS - 12

ER -