Stimulation of the type III olfactory adenylyl cyclase by calcium and calmodulin

Eui Ju Choi, Zhengui Xia, Daniel R. Storm

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

Characterization of adenylyl cyclases has been facilitated by the isolation of cDNA clones for distinct adenylyl cyclases including the type I and type III enzymes. Expression of type I adenylyl cyclase activity in animal cells has established that this enzyme is stimulated by calmodulin and Ca2+. Type III adenylyl cyclase is enriched in olfactory neurons and is regulated by stimulatory G proteins. The sensitivity of the type III adenylyl cyclase to Ca2+ and calmodulin has not been reported. In this study, type III adenylyl cyclase was expressed in human kidney 293 cells to determine if the enzyme is stimulated by Ca2+ and calmodulin. The type III enzyme was not stimulated by Ca2+ and calmodulin in the absence of other effectors. It was, however, stimulated by Ca2+ through calmodulin when the enzyme was concomitantly activated by either GppNHp or forskolin. The concentrations of free Ca2+ for half-maximal stimulation of type I and type III adenylyl cyclases were 0.05 and 5.0 μM Ca2+, respectively. These data suggest that the type-III adenylyl cyclase is stimulated by Ca2+ when the enzyme is activated by G-protein-coupled receptors and that increases in free Ca2+ accompanying receptor activation may amplify the primary cyclic AMP signal.

Original languageEnglish
Pages (from-to)6492-6498
Number of pages7
JournalBiochemistry
Volume31
Issue number28
Publication statusPublished - 1992 Dec 1
Externally publishedYes

Fingerprint

Calmodulin
Adenylyl Cyclases
Calcium
Enzymes
Colforsin
G-Protein-Coupled Receptors
GTP-Binding Proteins
Cyclic AMP
Neurons
adenylate cyclase 3
Animals
Complementary DNA
Clone Cells
Chemical activation
Cells
Kidney
adenylyl cyclase 1

ASJC Scopus subject areas

  • Biochemistry

Cite this

Stimulation of the type III olfactory adenylyl cyclase by calcium and calmodulin. / Choi, Eui Ju; Xia, Zhengui; Storm, Daniel R.

In: Biochemistry, Vol. 31, No. 28, 01.12.1992, p. 6492-6498.

Research output: Contribution to journalArticle

Choi, Eui Ju ; Xia, Zhengui ; Storm, Daniel R. / Stimulation of the type III olfactory adenylyl cyclase by calcium and calmodulin. In: Biochemistry. 1992 ; Vol. 31, No. 28. pp. 6492-6498.
@article{c37bb4832b7448c8b3427dbc90908837,
title = "Stimulation of the type III olfactory adenylyl cyclase by calcium and calmodulin",
abstract = "Characterization of adenylyl cyclases has been facilitated by the isolation of cDNA clones for distinct adenylyl cyclases including the type I and type III enzymes. Expression of type I adenylyl cyclase activity in animal cells has established that this enzyme is stimulated by calmodulin and Ca2+. Type III adenylyl cyclase is enriched in olfactory neurons and is regulated by stimulatory G proteins. The sensitivity of the type III adenylyl cyclase to Ca2+ and calmodulin has not been reported. In this study, type III adenylyl cyclase was expressed in human kidney 293 cells to determine if the enzyme is stimulated by Ca2+ and calmodulin. The type III enzyme was not stimulated by Ca2+ and calmodulin in the absence of other effectors. It was, however, stimulated by Ca2+ through calmodulin when the enzyme was concomitantly activated by either GppNHp or forskolin. The concentrations of free Ca2+ for half-maximal stimulation of type I and type III adenylyl cyclases were 0.05 and 5.0 μM Ca2+, respectively. These data suggest that the type-III adenylyl cyclase is stimulated by Ca2+ when the enzyme is activated by G-protein-coupled receptors and that increases in free Ca2+ accompanying receptor activation may amplify the primary cyclic AMP signal.",
author = "Choi, {Eui Ju} and Zhengui Xia and Storm, {Daniel R.}",
year = "1992",
month = "12",
day = "1",
language = "English",
volume = "31",
pages = "6492--6498",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "28",

}

TY - JOUR

T1 - Stimulation of the type III olfactory adenylyl cyclase by calcium and calmodulin

AU - Choi, Eui Ju

AU - Xia, Zhengui

AU - Storm, Daniel R.

PY - 1992/12/1

Y1 - 1992/12/1

N2 - Characterization of adenylyl cyclases has been facilitated by the isolation of cDNA clones for distinct adenylyl cyclases including the type I and type III enzymes. Expression of type I adenylyl cyclase activity in animal cells has established that this enzyme is stimulated by calmodulin and Ca2+. Type III adenylyl cyclase is enriched in olfactory neurons and is regulated by stimulatory G proteins. The sensitivity of the type III adenylyl cyclase to Ca2+ and calmodulin has not been reported. In this study, type III adenylyl cyclase was expressed in human kidney 293 cells to determine if the enzyme is stimulated by Ca2+ and calmodulin. The type III enzyme was not stimulated by Ca2+ and calmodulin in the absence of other effectors. It was, however, stimulated by Ca2+ through calmodulin when the enzyme was concomitantly activated by either GppNHp or forskolin. The concentrations of free Ca2+ for half-maximal stimulation of type I and type III adenylyl cyclases were 0.05 and 5.0 μM Ca2+, respectively. These data suggest that the type-III adenylyl cyclase is stimulated by Ca2+ when the enzyme is activated by G-protein-coupled receptors and that increases in free Ca2+ accompanying receptor activation may amplify the primary cyclic AMP signal.

AB - Characterization of adenylyl cyclases has been facilitated by the isolation of cDNA clones for distinct adenylyl cyclases including the type I and type III enzymes. Expression of type I adenylyl cyclase activity in animal cells has established that this enzyme is stimulated by calmodulin and Ca2+. Type III adenylyl cyclase is enriched in olfactory neurons and is regulated by stimulatory G proteins. The sensitivity of the type III adenylyl cyclase to Ca2+ and calmodulin has not been reported. In this study, type III adenylyl cyclase was expressed in human kidney 293 cells to determine if the enzyme is stimulated by Ca2+ and calmodulin. The type III enzyme was not stimulated by Ca2+ and calmodulin in the absence of other effectors. It was, however, stimulated by Ca2+ through calmodulin when the enzyme was concomitantly activated by either GppNHp or forskolin. The concentrations of free Ca2+ for half-maximal stimulation of type I and type III adenylyl cyclases were 0.05 and 5.0 μM Ca2+, respectively. These data suggest that the type-III adenylyl cyclase is stimulated by Ca2+ when the enzyme is activated by G-protein-coupled receptors and that increases in free Ca2+ accompanying receptor activation may amplify the primary cyclic AMP signal.

UR - http://www.scopus.com/inward/record.url?scp=0026681492&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026681492&partnerID=8YFLogxK

M3 - Article

C2 - 1633161

AN - SCOPUS:0026681492

VL - 31

SP - 6492

EP - 6498

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 28

ER -