STMN2 is a novel target of β-catenin/TCF-mediated transcription in human hepatoma cells

Heun Sik Lee, Dong Chul Lee, Mee Hee Park, Suk Jin Yang, Jung Ju Lee, Dong Min Kim, Yejin Jang, Jae Hyuck Lee, Jong Young Choi, Yun Kyung Kang, Dae Il Kim, Kyung Chan Park, Seon Young Kim, Hyang Sook Yoo, Eui Ju Choi, Young Il Yeom

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The activity of β-catenin/TCF, the key component of Wnt signaling pathway, is frequently deregulated in human cancers, resulting in the activation of genes whose dysregulation has significant consequences on tumor development. Therefore, identifying the target genes of Wnt signaling is important for understanding β-catenin-mediated carcinogenesis. Here, we report STMN2, a gene implicated in the regulation of microtubule dynamics, as a novel target of β-catenin-mediated transcription. STMN2 was up-regulated in hepatoma and cirrhotic liver tissues compared to normal liver and also in cell lines where β-catenin/TCF is constitutively activated. Transient activation of β-catenin/TCF either by transfection of a constitutively active form of β-catenin or by LiCl treatment induced the STMN2 mRNA expression in PLC/PRF/5 cells. Of the four members of STMN gene family, only STMN2 showed a Wnt-dependent expression pattern. Through promoter mapping and chromatin immunoprecipitation assays, we found that STMN2 is a direct target of β-catenin/TCF-mediated transcription and that the TCF binding site at -1713 of STMN2 promoter is critical for β-catenin/TCF-dependent expression regulation. siRNA-mediated knock-down of STMN2 expression indicated that STMN2 is required for maintaining the anchorage-independent growth state of β-catenin/TCF-activated hepatoma cells. Our results suggest that STMN2 might be a novel player of β-catenin/TCF-mediated carcinogenesis in the liver.

Original languageEnglish
Pages (from-to)1059-1067
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume345
Issue number3
DOIs
Publication statusPublished - 2006 Jul 7

Fingerprint

Catenins
Transcription
Hepatocellular Carcinoma
Genes
Liver
Carcinogenesis
Chemical activation
Wnt Signaling Pathway
Chromatin Immunoprecipitation
Programmable logic controllers
Microtubules
Small Interfering RNA
Transcriptional Activation
Chromatin
Transfection
Tumors
Assays
Neoplasms
Binding Sites
Cells

Keywords

  • β-Catenin/TCF
  • HCC
  • STMN2
  • Target genes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Lee, H. S., Lee, D. C., Park, M. H., Yang, S. J., Lee, J. J., Kim, D. M., ... Yeom, Y. I. (2006). STMN2 is a novel target of β-catenin/TCF-mediated transcription in human hepatoma cells. Biochemical and Biophysical Research Communications, 345(3), 1059-1067. https://doi.org/10.1016/j.bbrc.2006.05.017

STMN2 is a novel target of β-catenin/TCF-mediated transcription in human hepatoma cells. / Lee, Heun Sik; Lee, Dong Chul; Park, Mee Hee; Yang, Suk Jin; Lee, Jung Ju; Kim, Dong Min; Jang, Yejin; Lee, Jae Hyuck; Choi, Jong Young; Kang, Yun Kyung; Kim, Dae Il; Park, Kyung Chan; Kim, Seon Young; Yoo, Hyang Sook; Choi, Eui Ju; Yeom, Young Il.

In: Biochemical and Biophysical Research Communications, Vol. 345, No. 3, 07.07.2006, p. 1059-1067.

Research output: Contribution to journalArticle

Lee, HS, Lee, DC, Park, MH, Yang, SJ, Lee, JJ, Kim, DM, Jang, Y, Lee, JH, Choi, JY, Kang, YK, Kim, DI, Park, KC, Kim, SY, Yoo, HS, Choi, EJ & Yeom, YI 2006, 'STMN2 is a novel target of β-catenin/TCF-mediated transcription in human hepatoma cells', Biochemical and Biophysical Research Communications, vol. 345, no. 3, pp. 1059-1067. https://doi.org/10.1016/j.bbrc.2006.05.017
Lee, Heun Sik ; Lee, Dong Chul ; Park, Mee Hee ; Yang, Suk Jin ; Lee, Jung Ju ; Kim, Dong Min ; Jang, Yejin ; Lee, Jae Hyuck ; Choi, Jong Young ; Kang, Yun Kyung ; Kim, Dae Il ; Park, Kyung Chan ; Kim, Seon Young ; Yoo, Hyang Sook ; Choi, Eui Ju ; Yeom, Young Il. / STMN2 is a novel target of β-catenin/TCF-mediated transcription in human hepatoma cells. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 345, No. 3. pp. 1059-1067.
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AU - Lee, Dong Chul

AU - Park, Mee Hee

AU - Yang, Suk Jin

AU - Lee, Jung Ju

AU - Kim, Dong Min

AU - Jang, Yejin

AU - Lee, Jae Hyuck

AU - Choi, Jong Young

AU - Kang, Yun Kyung

AU - Kim, Dae Il

AU - Park, Kyung Chan

AU - Kim, Seon Young

AU - Yoo, Hyang Sook

AU - Choi, Eui Ju

AU - Yeom, Young Il

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N2 - The activity of β-catenin/TCF, the key component of Wnt signaling pathway, is frequently deregulated in human cancers, resulting in the activation of genes whose dysregulation has significant consequences on tumor development. Therefore, identifying the target genes of Wnt signaling is important for understanding β-catenin-mediated carcinogenesis. Here, we report STMN2, a gene implicated in the regulation of microtubule dynamics, as a novel target of β-catenin-mediated transcription. STMN2 was up-regulated in hepatoma and cirrhotic liver tissues compared to normal liver and also in cell lines where β-catenin/TCF is constitutively activated. Transient activation of β-catenin/TCF either by transfection of a constitutively active form of β-catenin or by LiCl treatment induced the STMN2 mRNA expression in PLC/PRF/5 cells. Of the four members of STMN gene family, only STMN2 showed a Wnt-dependent expression pattern. Through promoter mapping and chromatin immunoprecipitation assays, we found that STMN2 is a direct target of β-catenin/TCF-mediated transcription and that the TCF binding site at -1713 of STMN2 promoter is critical for β-catenin/TCF-dependent expression regulation. siRNA-mediated knock-down of STMN2 expression indicated that STMN2 is required for maintaining the anchorage-independent growth state of β-catenin/TCF-activated hepatoma cells. Our results suggest that STMN2 might be a novel player of β-catenin/TCF-mediated carcinogenesis in the liver.

AB - The activity of β-catenin/TCF, the key component of Wnt signaling pathway, is frequently deregulated in human cancers, resulting in the activation of genes whose dysregulation has significant consequences on tumor development. Therefore, identifying the target genes of Wnt signaling is important for understanding β-catenin-mediated carcinogenesis. Here, we report STMN2, a gene implicated in the regulation of microtubule dynamics, as a novel target of β-catenin-mediated transcription. STMN2 was up-regulated in hepatoma and cirrhotic liver tissues compared to normal liver and also in cell lines where β-catenin/TCF is constitutively activated. Transient activation of β-catenin/TCF either by transfection of a constitutively active form of β-catenin or by LiCl treatment induced the STMN2 mRNA expression in PLC/PRF/5 cells. Of the four members of STMN gene family, only STMN2 showed a Wnt-dependent expression pattern. Through promoter mapping and chromatin immunoprecipitation assays, we found that STMN2 is a direct target of β-catenin/TCF-mediated transcription and that the TCF binding site at -1713 of STMN2 promoter is critical for β-catenin/TCF-dependent expression regulation. siRNA-mediated knock-down of STMN2 expression indicated that STMN2 is required for maintaining the anchorage-independent growth state of β-catenin/TCF-activated hepatoma cells. Our results suggest that STMN2 might be a novel player of β-catenin/TCF-mediated carcinogenesis in the liver.

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