Stromal vascular fraction from adipose tissue forms profound vascular network through the dynamic reassembly of blood endothelial cells

Young Jun Koh, Bong Ihn Koh, Honsoul Kim, Hyung Joon Joo, Ho Kyoung Jin, Jongwook Jeon, Chulhee Choi, Dong Hun Lee, Jin Ho Chung, Chung Hyun Cho, Won Seok Park, Ji Kan Ryu, Jun Kyu Suh, Gou Young Koh

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82 Citations (Scopus)

Abstract

Objective- Tremendous efforts have been made to establish effective therapeutic neovascularization using adipose tissue-derived stromal vascular fraction (SVF), but the efficiency is low, and underlying mechanisms and their interaction with the host in a new microenvironment are poorly understood. Methods and Results- Here we demonstrate that direct implantation of SVF derived from donor adipose tissue can create a profound vascular network through the disassembly and reassembly of blood endothelial cells at the site of implantation. This neovasculature successfully established connection with recipient blood vessels to form a functionally perfused circuit. Addition of vascular growth factors to the SVF implant improved the efficiency of functional neovasculature formation. In contrast, spheroid culture of SVF before implantation reduced the capacity of vasculature formation, possibly because of cellular alteration. Implanting SVF into the mouse ischemic hindlimb induced the robust formation of a local neovascular network and salvaged the limb. Moreover, the coimplantation of SVF prevented fat absorption in the subcutaneous adipose tissue graft model. Conclusion- Freshly isolated SVF can effectively induce new vessel formation through the dynamic reassembly of blood endothelial cells and could be applied to achieve therapeutic neovascularization for relieving ischemia and preventing fat absorption in an autologous manner.

Original languageEnglish
Pages (from-to)1141-1150
Number of pages10
JournalArteriosclerosis, thrombosis, and vascular biology
Volume31
Issue number5
DOIs
Publication statusPublished - 2011 May

Keywords

  • angiogenesis
  • ischemia
  • vascular biology

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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    Koh, Y. J., Koh, B. I., Kim, H., Joo, H. J., Jin, H. K., Jeon, J., Choi, C., Lee, D. H., Chung, J. H., Cho, C. H., Park, W. S., Ryu, J. K., Suh, J. K., & Koh, G. Y. (2011). Stromal vascular fraction from adipose tissue forms profound vascular network through the dynamic reassembly of blood endothelial cells. Arteriosclerosis, thrombosis, and vascular biology, 31(5), 1141-1150. https://doi.org/10.1161/ATVBAHA.110.218206