Abstract
Objective- Tremendous efforts have been made to establish effective therapeutic neovascularization using adipose tissue-derived stromal vascular fraction (SVF), but the efficiency is low, and underlying mechanisms and their interaction with the host in a new microenvironment are poorly understood. Methods and Results- Here we demonstrate that direct implantation of SVF derived from donor adipose tissue can create a profound vascular network through the disassembly and reassembly of blood endothelial cells at the site of implantation. This neovasculature successfully established connection with recipient blood vessels to form a functionally perfused circuit. Addition of vascular growth factors to the SVF implant improved the efficiency of functional neovasculature formation. In contrast, spheroid culture of SVF before implantation reduced the capacity of vasculature formation, possibly because of cellular alteration. Implanting SVF into the mouse ischemic hindlimb induced the robust formation of a local neovascular network and salvaged the limb. Moreover, the coimplantation of SVF prevented fat absorption in the subcutaneous adipose tissue graft model. Conclusion- Freshly isolated SVF can effectively induce new vessel formation through the dynamic reassembly of blood endothelial cells and could be applied to achieve therapeutic neovascularization for relieving ischemia and preventing fat absorption in an autologous manner.
| Original language | English |
|---|---|
| Pages (from-to) | 1141-1150 |
| Number of pages | 10 |
| Journal | Arteriosclerosis, Thrombosis, and Vascular Biology |
| Volume | 31 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2011 May 1 |
| Externally published | Yes |
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Keywords
- angiogenesis
- ischemia
- vascular biology
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Stromal vascular fraction from adipose tissue forms profound vascular network through the dynamic reassembly of blood endothelial cells. / Koh, Young Jun; Koh, Bong Ihn; Kim, Honsoul; Joo, Hyung Joon; Jin, Ho Kyoung; Jeon, Jongwook; Choi, Chulhee; Lee, Dong Hun; Chung, Jin Ho; Cho, Chung Hyun; Park, Won Seok; Ryu, Ji Kan; Suh, Jun Kyu; Koh, Gou Young.
In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 31, No. 5, 01.05.2011, p. 1141-1150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stromal vascular fraction from adipose tissue forms profound vascular network through the dynamic reassembly of blood endothelial cells
AU - Koh, Young Jun
AU - Koh, Bong Ihn
AU - Kim, Honsoul
AU - Joo, Hyung Joon
AU - Jin, Ho Kyoung
AU - Jeon, Jongwook
AU - Choi, Chulhee
AU - Lee, Dong Hun
AU - Chung, Jin Ho
AU - Cho, Chung Hyun
AU - Park, Won Seok
AU - Ryu, Ji Kan
AU - Suh, Jun Kyu
AU - Koh, Gou Young
PY - 2011/5/1
Y1 - 2011/5/1
N2 - Objective- Tremendous efforts have been made to establish effective therapeutic neovascularization using adipose tissue-derived stromal vascular fraction (SVF), but the efficiency is low, and underlying mechanisms and their interaction with the host in a new microenvironment are poorly understood. Methods and Results- Here we demonstrate that direct implantation of SVF derived from donor adipose tissue can create a profound vascular network through the disassembly and reassembly of blood endothelial cells at the site of implantation. This neovasculature successfully established connection with recipient blood vessels to form a functionally perfused circuit. Addition of vascular growth factors to the SVF implant improved the efficiency of functional neovasculature formation. In contrast, spheroid culture of SVF before implantation reduced the capacity of vasculature formation, possibly because of cellular alteration. Implanting SVF into the mouse ischemic hindlimb induced the robust formation of a local neovascular network and salvaged the limb. Moreover, the coimplantation of SVF prevented fat absorption in the subcutaneous adipose tissue graft model. Conclusion- Freshly isolated SVF can effectively induce new vessel formation through the dynamic reassembly of blood endothelial cells and could be applied to achieve therapeutic neovascularization for relieving ischemia and preventing fat absorption in an autologous manner.
AB - Objective- Tremendous efforts have been made to establish effective therapeutic neovascularization using adipose tissue-derived stromal vascular fraction (SVF), but the efficiency is low, and underlying mechanisms and their interaction with the host in a new microenvironment are poorly understood. Methods and Results- Here we demonstrate that direct implantation of SVF derived from donor adipose tissue can create a profound vascular network through the disassembly and reassembly of blood endothelial cells at the site of implantation. This neovasculature successfully established connection with recipient blood vessels to form a functionally perfused circuit. Addition of vascular growth factors to the SVF implant improved the efficiency of functional neovasculature formation. In contrast, spheroid culture of SVF before implantation reduced the capacity of vasculature formation, possibly because of cellular alteration. Implanting SVF into the mouse ischemic hindlimb induced the robust formation of a local neovascular network and salvaged the limb. Moreover, the coimplantation of SVF prevented fat absorption in the subcutaneous adipose tissue graft model. Conclusion- Freshly isolated SVF can effectively induce new vessel formation through the dynamic reassembly of blood endothelial cells and could be applied to achieve therapeutic neovascularization for relieving ischemia and preventing fat absorption in an autologous manner.
KW - angiogenesis
KW - ischemia
KW - vascular biology
UR - http://www.scopus.com/inward/record.url?scp=79955603409&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955603409&partnerID=8YFLogxK
U2 - 10.1161/ATVBAHA.110.218206
DO - 10.1161/ATVBAHA.110.218206
M3 - Article
C2 - 21393582
AN - SCOPUS:79955603409
VL - 31
SP - 1141
EP - 1150
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
SN - 1079-5642
IS - 5
ER -