Strong alkaline condition is preferable for producing 7,10-dihydroxy-8(E)-hexadecenoic acid from palmitoleic acid by Pseudomonas aeruginosa KNU-2B

Ji Sun Moon, Yeon Jung Lee, In Hwan Kim, Hak Ryul Kim

Research output: Contribution to journalArticle

Abstract

Microbial bioconversion of a given substrate is considered an efficient and eco-friendly tool for value-added industrial compound generation from natural products. Among natural products, unsaturated fatty acids have been used as substrates to produce various functional hydroxy fatty acids. In this study, we report the production of 7,10-dihydroxy-8(E)-hexadecenoic acid (DHD) from palmitoleic acid by a new strain, Pseudomonas aeruginosa KNU-2B. KNU-2B efficiently produced DHD from palmitoleic acid and required a strong alkaline condition for maximum DHD production. The maximum DHD amount produced under pH 10.0 and 48-h incubation at 27 °C and 150 rpm was 219.5 mg/100 mL culture. Other important nutritional factors were also investigated to obtain optimum DHD production.

Original languageEnglish
Pages (from-to)1755-1766
Number of pages12
JournalBiotechnology letters
Volume42
Issue number9
DOIs
Publication statusPublished - 2020 Sep 1

Keywords

  • Alkaline condition
  • Bioconversion
  • Dihydroxy fatty acid
  • Palmitoleic acid
  • Pseudomonas aeruginosa KNU-2B

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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