Structural analysis and serological test of arginine periplasmic binding protein 2 from Chlamydophila pneumoniae

Sung Ha Park, Ji Eun Chang, Hye Jin Kim Hawkes, Yeon Ho Kang, Kwang Yeon Hwang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The 'art' genes encode specific arginine uptake proteins, and are repressed by the repressible promoters of ArgR, affecting transcription of artJ [1,2]. Cpb0502, the arginine-binding periplasmic protein 2 precursor from Chlamydophila pneumoniae TW-183 strains, is responsible for arginine transport. As C. pneumoniae is difficult to isolate and culture, there have been many studies of better ways to detect it. A microimmunofluorescence assay (MIF) is still considered to be the 'gold standard' for detecting C. pneumoniae. Although MIF has its own limitations, a number of immunogenic antigens have been shown to be C. pneumoniae specific by this test. Here, we report Cpb0502 as a specific immunogenic antigen against C. pneumoniae as it was detected only in human infection sera of C. pneumoniae but not in Legionella pneumophila and Mycoplasma pneumoniae infection sera, showing high specificity and sensitivity by MIF, western blot and ELISA analysis. And also the crystal structure of Cpb0502 was determined to be a dimer at 2.07. å, revealing a similar backbone structure to a histidine kinase receptor, HK29S. Therefore we may suggest that Cpb0502 is a candidate immunogenic antigen for better diagnosis of C. pneumoniae.

Original languageEnglish
Pages (from-to)518-524
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume418
Issue number3
DOIs
Publication statusPublished - 2012 Feb 17

Fingerprint

Periplasmic Binding Proteins
Chlamydophila pneumoniae
Serologic Tests
Structural analysis
Arginine
Assays
Antigens
Transcription
Histidine
Dimers
Phosphotransferases
Genes
Crystal structure
Legionella pneumophila
Mycoplasma pneumoniae
Protein Precursors
Art
Infection
Serum
Proteins

Keywords

  • Arginine periplasmic binding protein 2
  • Chlamydophila pneumoniae
  • Diagnosis
  • Immunogenic antigen
  • Serological test
  • Structural analysis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Structural analysis and serological test of arginine periplasmic binding protein 2 from Chlamydophila pneumoniae. / Park, Sung Ha; Chang, Ji Eun; Hawkes, Hye Jin Kim; Kang, Yeon Ho; Hwang, Kwang Yeon.

In: Biochemical and Biophysical Research Communications, Vol. 418, No. 3, 17.02.2012, p. 518-524.

Research output: Contribution to journalArticle

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