Structural analysis of immunostimulating sulfated polysaccharides from Ulva pertusa

Mehdi Tabarsa, Sung Joon Lee, Sangguan You

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)


Sulfated polysaccharides were extracted from Ulva pertusa and fractionated to obtain the most immunostimulating fraction (F2). The glycosidic linkages of the polysaccharides in the fraction F2 were determined using GC-MS and 2D-NMR spectroscopy after chemical modifications, including reduction and desulfation under various conditions. Methanol was used as a sulfate acceptor for the removal of sulfates from the polysaccharides. When desulfation was carried out at 120°C, the sulfates were removed upto 90.1% from the F2 fraction without considerable backbone degradation. The GC-MS analysis as well as NMR spectra revealed that the backbone of the polysaccharides was mainly composed of α-(1→4)-l-rhamnopyranosyl, β-(1→4)-d-glucuronosyl, β-(1→2)-l-rhamnopyranosyl, and β-(1→4)-d-xylopyranosyl residues with branches at O-2 position of rhamnose. The sulfate groups were mostly found on glucuronic acid at O-3 position.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournalCarbohydrate Research
Publication statusPublished - 2012 Nov 1


  • 2D-NMR
  • GC-MS
  • Glycosidic linkages
  • Sulfated polysaccharide
  • Ulva pertusa

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


Dive into the research topics of 'Structural analysis of immunostimulating sulfated polysaccharides from Ulva pertusa'. Together they form a unique fingerprint.

Cite this