Abstract
Sulfated polysaccharides were extracted from Ulva pertusa and fractionated to obtain the most immunostimulating fraction (F2). The glycosidic linkages of the polysaccharides in the fraction F2 were determined using GC-MS and 2D-NMR spectroscopy after chemical modifications, including reduction and desulfation under various conditions. Methanol was used as a sulfate acceptor for the removal of sulfates from the polysaccharides. When desulfation was carried out at 120°C, the sulfates were removed upto 90.1% from the F2 fraction without considerable backbone degradation. The GC-MS analysis as well as NMR spectra revealed that the backbone of the polysaccharides was mainly composed of α-(1→4)-l-rhamnopyranosyl, β-(1→4)-d-glucuronosyl, β-(1→2)-l-rhamnopyranosyl, and β-(1→4)-d-xylopyranosyl residues with branches at O-2 position of rhamnose. The sulfate groups were mostly found on glucuronic acid at O-3 position.
| Original language | English |
|---|---|
| Pages (from-to) | 141-147 |
| Number of pages | 7 |
| Journal | Carbohydrate Research |
| Volume | 361 |
| DOIs | |
| Publication status | Published - 2012 Nov 1 |
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Keywords
- 2D-NMR
- GC-MS
- Glycosidic linkages
- Sulfated polysaccharide
- Ulva pertusa
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Organic Chemistry
Cite this
Structural analysis of immunostimulating sulfated polysaccharides from Ulva pertusa. / Tabarsa, Mehdi; Lee, Sung-Joon; You, Sangguan.
In: Carbohydrate Research, Vol. 361, 01.11.2012, p. 141-147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structural analysis of immunostimulating sulfated polysaccharides from Ulva pertusa
AU - Tabarsa, Mehdi
AU - Lee, Sung-Joon
AU - You, Sangguan
PY - 2012/11/1
Y1 - 2012/11/1
N2 - Sulfated polysaccharides were extracted from Ulva pertusa and fractionated to obtain the most immunostimulating fraction (F2). The glycosidic linkages of the polysaccharides in the fraction F2 were determined using GC-MS and 2D-NMR spectroscopy after chemical modifications, including reduction and desulfation under various conditions. Methanol was used as a sulfate acceptor for the removal of sulfates from the polysaccharides. When desulfation was carried out at 120°C, the sulfates were removed upto 90.1% from the F2 fraction without considerable backbone degradation. The GC-MS analysis as well as NMR spectra revealed that the backbone of the polysaccharides was mainly composed of α-(1→4)-l-rhamnopyranosyl, β-(1→4)-d-glucuronosyl, β-(1→2)-l-rhamnopyranosyl, and β-(1→4)-d-xylopyranosyl residues with branches at O-2 position of rhamnose. The sulfate groups were mostly found on glucuronic acid at O-3 position.
AB - Sulfated polysaccharides were extracted from Ulva pertusa and fractionated to obtain the most immunostimulating fraction (F2). The glycosidic linkages of the polysaccharides in the fraction F2 were determined using GC-MS and 2D-NMR spectroscopy after chemical modifications, including reduction and desulfation under various conditions. Methanol was used as a sulfate acceptor for the removal of sulfates from the polysaccharides. When desulfation was carried out at 120°C, the sulfates were removed upto 90.1% from the F2 fraction without considerable backbone degradation. The GC-MS analysis as well as NMR spectra revealed that the backbone of the polysaccharides was mainly composed of α-(1→4)-l-rhamnopyranosyl, β-(1→4)-d-glucuronosyl, β-(1→2)-l-rhamnopyranosyl, and β-(1→4)-d-xylopyranosyl residues with branches at O-2 position of rhamnose. The sulfate groups were mostly found on glucuronic acid at O-3 position.
KW - 2D-NMR
KW - GC-MS
KW - Glycosidic linkages
KW - Sulfated polysaccharide
KW - Ulva pertusa
UR - http://www.scopus.com/inward/record.url?scp=84866528796&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866528796&partnerID=8YFLogxK
U2 - 10.1016/j.carres.2012.09.006
DO - 10.1016/j.carres.2012.09.006
M3 - Article
C2 - 23023040
AN - SCOPUS:84866528796
VL - 361
SP - 141
EP - 147
JO - Carbohydrate Research
JF - Carbohydrate Research
SN - 0008-6215
ER -