Structural and biochemical characterization of FabK from Thermotoga maritima

Byung Hak Ha, Sang Chul Shin, Jin Ho Moon, Gyochang Keum, Chan Wha Kim, Eunice Eun Kyeong Kim

Research output: Contribution to journalArticle

Abstract

TM0800 from Thermotoga maritima is one of the hypothetical proteins with unknown function. The crystal structure determined at 2.3 Å resolution reveals a two domain structure: the N-terminal domain forming a barrel and the C-terminal forming a lid. One FMN is bound between the two domains with the phosphate making intricate hydrogen bonds with protein and three tightly bound water molecules, and the isoalloxazine ring packed against the side chains of Met22 and Met276. The structure is almost identical to that of FabK (enoyl-acyl carrier protein (ACP) reductase, ENR II), a key enzyme in bacterial type II fatty-acid biosynthesis that catalyzes the final step in each elongation cycle; and the enzymatic activity confirms that TM0800 is an ENR. Enzymatic activity was almost completely abolished when the helices connecting the barrel and the lid were deleted. Also, the Met276Ala and Ser280Ala mutants showed a significant reduction in enzymatic activity. The crystal structure of Met276Ala mutant at 1.9 Å resolution showed an absence of FMN suggesting that FMN plays a role in catalysis, and Met276 is important in positioning FMN. TmFabK exists as a dimer in both solution and crystal. Together this study provides molecular basis for the catalytic activity of FabK.

Original languageEnglish
Pages (from-to)968-974
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume482
Issue number4
DOIs
Publication statusPublished - 2017 Jan 22

Fingerprint

Thermotoga maritima
Flavin Mononucleotide
Crystal structure
Acyl Carrier Protein
Biosynthesis
Catalysis
Dimers
Elongation
Hydrogen
Catalyst activity
Hydrogen bonds
Oxidoreductases
Proteins
Fatty Acids
Phosphates
Crystals
Molecules
Water
Enzymes

Keywords

  • Biochemical characterization
  • Crystal structure
  • Enoyl-acyl carrier protein (ACP) reductase
  • FabK

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Structural and biochemical characterization of FabK from Thermotoga maritima. / Ha, Byung Hak; Shin, Sang Chul; Moon, Jin Ho; Keum, Gyochang; Kim, Chan Wha; Kim, Eunice Eun Kyeong.

In: Biochemical and Biophysical Research Communications, Vol. 482, No. 4, 22.01.2017, p. 968-974.

Research output: Contribution to journalArticle

Ha, Byung Hak ; Shin, Sang Chul ; Moon, Jin Ho ; Keum, Gyochang ; Kim, Chan Wha ; Kim, Eunice Eun Kyeong. / Structural and biochemical characterization of FabK from Thermotoga maritima. In: Biochemical and Biophysical Research Communications. 2017 ; Vol. 482, No. 4. pp. 968-974.
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