Abstract
Hormone-sensitive lipase (HSL) plays an important role in the regulation of rodent fat cell lipolysis. It is regarded as an adipose tissue-specific enzyme whose sole metabolic role is the catalysis of hormone-stimulated lipolysis in mammalian cells. In this report we describe the functional and structural analysis of an EstE5 protein from a soil metagenome library. Function analysis results indicated that EstE5 preferentially hydrolyzes short-chain ester compounds, and our kinetic studies revealed the optimal pH and temperature. Based on the structural analysis, we defined the active site and the binding pocket. Structurally, EstE5 belongs to the HSL family and these structural studies may have applications in the production of value-added products, including pharmaceuticals.
| Original language | English |
|---|---|
| Pages (from-to) | 553-556 |
| Number of pages | 4 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 379 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 Feb 6 |
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Keywords
- Crystal structure
- EstE5
- Esterase/lipase
- Hormone-sensitive lipase
- HSL family
- Soil metagenome library
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
Cite this
Structural and functional analysis of a novel EstE5 belonging to the subfamily of hormone-sensitive lipase. / Nam, Ki Hyun; Kim, Min Young; Kim, Soo Jin; Priyadarshi, Amit; Lee, Won Ho; Hwang, Kwang Yeon.
In: Biochemical and Biophysical Research Communications, Vol. 379, No. 2, 06.02.2009, p. 553-556.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structural and functional analysis of a novel EstE5 belonging to the subfamily of hormone-sensitive lipase
AU - Nam, Ki Hyun
AU - Kim, Min Young
AU - Kim, Soo Jin
AU - Priyadarshi, Amit
AU - Lee, Won Ho
AU - Hwang, Kwang Yeon
PY - 2009/2/6
Y1 - 2009/2/6
N2 - Hormone-sensitive lipase (HSL) plays an important role in the regulation of rodent fat cell lipolysis. It is regarded as an adipose tissue-specific enzyme whose sole metabolic role is the catalysis of hormone-stimulated lipolysis in mammalian cells. In this report we describe the functional and structural analysis of an EstE5 protein from a soil metagenome library. Function analysis results indicated that EstE5 preferentially hydrolyzes short-chain ester compounds, and our kinetic studies revealed the optimal pH and temperature. Based on the structural analysis, we defined the active site and the binding pocket. Structurally, EstE5 belongs to the HSL family and these structural studies may have applications in the production of value-added products, including pharmaceuticals.
AB - Hormone-sensitive lipase (HSL) plays an important role in the regulation of rodent fat cell lipolysis. It is regarded as an adipose tissue-specific enzyme whose sole metabolic role is the catalysis of hormone-stimulated lipolysis in mammalian cells. In this report we describe the functional and structural analysis of an EstE5 protein from a soil metagenome library. Function analysis results indicated that EstE5 preferentially hydrolyzes short-chain ester compounds, and our kinetic studies revealed the optimal pH and temperature. Based on the structural analysis, we defined the active site and the binding pocket. Structurally, EstE5 belongs to the HSL family and these structural studies may have applications in the production of value-added products, including pharmaceuticals.
KW - Crystal structure
KW - EstE5
KW - Esterase/lipase
KW - Hormone-sensitive lipase
KW - HSL family
KW - Soil metagenome library
UR - http://www.scopus.com/inward/record.url?scp=58149525330&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149525330&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2008.12.085
DO - 10.1016/j.bbrc.2008.12.085
M3 - Article
C2 - 19116143
AN - SCOPUS:58149525330
VL - 379
SP - 553
EP - 556
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 2
ER -