Structural and functional analysis of Vitamin K2 synthesis protein MenD

Amit Priyadarshi, Eunice Eun Kyeong Kim, Kwang Yeon Hwang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Here we describe in detail the crystal structures of the Vitamin K2 synthesis protein MenD, from Escherichia coli, in complex with thiamine diphosphate (ThDP) and oxoglutarate, and the effects of cofactor and substrate on its structural stability. This is the first reported structure of MenD in complex with oxoglutarate. The residues Gly472 to Phe488 of the active site region are either disordered, or in an open conformation in the MenD oxoglutarate complex structure, but adopt a closed conformation in the MenD ThDP complex structure. Biospecific-interaction analysis using surface plasmon resonance (SPR) technology reveals an affinity for ThDP and oxoglutarate in the nanomolar range. Biochemical and structural analysis confirmed that MenD is highly dependent on ThDP for its structural stability. Our structural results combined with the biochemical assay reveal novel features of the enzyme that could be utilized in a program of rational structure-based drug design, as well as in helping to enhance our knowledge of the menaquinone synthesis pathway in greater detail.

Original languageEnglish
Pages (from-to)748-751
Number of pages4
JournalBiochemical and biophysical research communications
Volume388
Issue number4
DOIs
Publication statusPublished - 2009 Oct 30

Keywords

  • MenD
  • Menaquinone
  • Oxoglutarate
  • SPR
  • ThDP
  • Vitamin K

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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