TY - JOUR
T1 - Structural and immunological feature of rhamnogalacturonan I-rich polysaccharide from Korean persimmon vinegar
AU - Kim, Hoon
AU - Hong, Hee Do
AU - Suh, Hyung Joo
AU - Shin, Kwang Soon
N1 - Funding Information:
This study was supported by The Globalization of Korean Foods R&D Program of the Ministry of Agriculture and Food and Rural Affairs . Also, a part of this study was supported by a grant from the Korean Food Research Institute (2015). We express our sincere gratitude for their supports.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - The crude polysaccharide (KPV-0) isolated from Korean persimmon vinegar was fractionated using gel filtration chromatography to enhance the immunostimulatory activity and to identify the structural features of active fraction. Among three fractions, KPV-I obtained in a void volume, demonstrated the potent production of macrophage-stimulating mediators, including tumor necrosis factor-α, interleukin (IL)-6, IL-12, and nitric oxide. KPV-I showed a combined single peak with high molecular weight of 55,000 Da by high performance size exclusion chromatography. Component sugar analysis revealed that KPV-I contained mainly of arabinose, mannose, galactose, rhamnose and galacturonic acid. Single radial gel diffusion assay using β-glucosyl Yariv reagent showed that KPV-I contained arabinogalactan protein with 13.7%. Methylation analysis indicated that KPV-I contained 21 kinds of neutral glycosidic linkages, which seemed to be composed three kinds of polysaccharide; that is a rhamnogalacturonan-I (65–70%) derived from persimmon as a raw material, a mannan (20–25%) derived from fermentation-associated microorganisms, and a linear glucans (less than 10%). In conclusion, polysaccharide isolated from persimmon vinegar could augment the macrophage stimulation, and a large amounts of RG-I polysaccharide derived from persimmon is likely a crucial role in expression of the activity in persimmon vinegar.
AB - The crude polysaccharide (KPV-0) isolated from Korean persimmon vinegar was fractionated using gel filtration chromatography to enhance the immunostimulatory activity and to identify the structural features of active fraction. Among three fractions, KPV-I obtained in a void volume, demonstrated the potent production of macrophage-stimulating mediators, including tumor necrosis factor-α, interleukin (IL)-6, IL-12, and nitric oxide. KPV-I showed a combined single peak with high molecular weight of 55,000 Da by high performance size exclusion chromatography. Component sugar analysis revealed that KPV-I contained mainly of arabinose, mannose, galactose, rhamnose and galacturonic acid. Single radial gel diffusion assay using β-glucosyl Yariv reagent showed that KPV-I contained arabinogalactan protein with 13.7%. Methylation analysis indicated that KPV-I contained 21 kinds of neutral glycosidic linkages, which seemed to be composed three kinds of polysaccharide; that is a rhamnogalacturonan-I (65–70%) derived from persimmon as a raw material, a mannan (20–25%) derived from fermentation-associated microorganisms, and a linear glucans (less than 10%). In conclusion, polysaccharide isolated from persimmon vinegar could augment the macrophage stimulation, and a large amounts of RG-I polysaccharide derived from persimmon is likely a crucial role in expression of the activity in persimmon vinegar.
KW - Macrophage stimulation
KW - Mannan
KW - Persimmon vinegar
KW - Polysaccharide
KW - Rhamnogalacturonan I
UR - http://www.scopus.com/inward/record.url?scp=84981727673&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2016.04.060
DO - 10.1016/j.ijbiomac.2016.04.060
M3 - Article
C2 - 27131731
AN - SCOPUS:84981727673
SN - 0141-8130
VL - 89
SP - 319
EP - 327
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -