Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes revealed by high-resolution X-ray crystallography

Gye Won Han, Jae Young Lee, Hyun Kyu Song, Changsoo Chang, Kyeongsik Min, Jinho Moon, Dong Hae Shin, Mary L. Kopka, Michael R. Sawaya, Hanna S. Yuan, Thomas D. Kim, Jungwoo Choe, Dori Lim, Hee Jung Moon, Se Won Suh

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Non-specific lipid-transfer proteins (nsLTPs) are involved in the movement of phospholipids, glycolipids, fatty acids, and steroids between membranes. Several structures of plant nsLTPs have been determined both by X-ray crystallography and nuclear magnetic resonance. However, the detailed structural basis of the non-specific binding of hydrophobic ligands by nsLTPs is still poorly understood. In order to gain a better understanding of the structural basis of the non-specific binding of hydrophobic ligands by nsLTPs and to investigate the plasticity of the fatty acid binding cavity in nsLTPs, seven high-resolution (between 1.3 Å and 1.9 Å) crystal structures have been determined. These depict the nsLTP from maize seedlings in complex with an array of fatty acids. A detailed comparison of the structures of maize nsLTP in complex with various ligands reveals a new binding mode in an nsLTP-oleate complex which has not been seen before. Furthermore, in the caprate complex, the ligand binds to the protein cavity in two orientations with equal occupancy. The volume of the hydrophobic cavity in the nsLTP from maize shows some variation depending on the size of the bound ligands. The structural plasticity of the ligand binding cavity and the predominant involvement of non-specific van der Waals interactions with the hydrophobic tail of the ligands provide a structural explanation for the non-specificity of maize nsLTP. The hydrophobic cavity accommodates various ligands from C10 to C18. The C18:1 ricinoleate with its hydroxyl group hydrogen bonding to Ala68 possibly mimics cutin monomer binding which is of biological importance. Some of the myristate binding sites in human serum albumin resemble the maize nsLTP, implying the importance of a helical bundle in accommodating the non-specific binding of fatty acids.

Original languageEnglish
Pages (from-to)263-278
Number of pages16
JournalJournal of Molecular Biology
Volume308
Issue number2
DOIs
Publication statusPublished - 2001 Apr 27
Externally publishedYes

Fingerprint

X Ray Crystallography
Zea mays
Ligands
Lipids
Fatty Acids
Plant Structures
lipid transfer protein
Glycolipids
Myristic Acid
Hydrogen Bonding
Oleic Acid
Hydrophobic and Hydrophilic Interactions
Seedlings
Serum Albumin
Hydroxyl Radical
Tail
Phospholipids
Magnetic Resonance Spectroscopy
Steroids
Binding Sites

Keywords

  • Antifungal activity
  • Fatty acid binding
  • Hydrophobic cavity
  • Non-specific lipid-transfer protein
  • Structural plasticity

ASJC Scopus subject areas

  • Virology

Cite this

Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes revealed by high-resolution X-ray crystallography. / Han, Gye Won; Lee, Jae Young; Song, Hyun Kyu; Chang, Changsoo; Min, Kyeongsik; Moon, Jinho; Shin, Dong Hae; Kopka, Mary L.; Sawaya, Michael R.; Yuan, Hanna S.; Kim, Thomas D.; Choe, Jungwoo; Lim, Dori; Moon, Hee Jung; Suh, Se Won.

In: Journal of Molecular Biology, Vol. 308, No. 2, 27.04.2001, p. 263-278.

Research output: Contribution to journalArticle

Han, GW, Lee, JY, Song, HK, Chang, C, Min, K, Moon, J, Shin, DH, Kopka, ML, Sawaya, MR, Yuan, HS, Kim, TD, Choe, J, Lim, D, Moon, HJ & Suh, SW 2001, 'Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes revealed by high-resolution X-ray crystallography', Journal of Molecular Biology, vol. 308, no. 2, pp. 263-278. https://doi.org/10.1006/jmbi.2001.4559
Han, Gye Won ; Lee, Jae Young ; Song, Hyun Kyu ; Chang, Changsoo ; Min, Kyeongsik ; Moon, Jinho ; Shin, Dong Hae ; Kopka, Mary L. ; Sawaya, Michael R. ; Yuan, Hanna S. ; Kim, Thomas D. ; Choe, Jungwoo ; Lim, Dori ; Moon, Hee Jung ; Suh, Se Won. / Structural basis of non-specific lipid binding in maize lipid-transfer protein complexes revealed by high-resolution X-ray crystallography. In: Journal of Molecular Biology. 2001 ; Vol. 308, No. 2. pp. 263-278.
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