Structural Characterization of RNA Recognition Motif-2 Domain of SART3

Kyeong Mi Bang, Na Youn Cho, Won Je Kim, Ae Ryung Kim, Hyun Kyu Song, Eunice Eun Kyeong Kim, Nak Kyoon Kim

Research output: Contribution to journalArticle

Abstract

Squamous cell carcinoma antigen recognized by T-cells 3 (SART3) is an essential recycling factor in pre-mRNA splicing, which is required for association of U4/U6 small nuclear ribonucleoprotein (snRNP). SART3 contains two RNA recognition motifs (RRMs), and they are responsible for the tertiary interaction with U6 small nuclear RNA. Despite the importance of structural studies for understanding complicate U4/U6 snRNP recycling mechanism, only a few of them have been performed for SART3. Here, the structure of SART3 RRM2 was characterized by heteronuclear multi-dimensional nuclear magnetic resonance experiments. Nearly complete 1H, 15N, and 13C chemical shifts of the backbone residues of RRM2 were assigned. In addition, the secondary structure of RRM2 were predicted by the chemical shift index and TALOS+ analyses, and the results showed that RRM2 forms a “β1123245” structure, where β4 is not common in the canonical RRM domain structures. Our results will provide structural basis for investigation of SART3-mediated U4/U6 snRNP complex formation.

Original languageEnglish
Pages (from-to)444-447
Number of pages4
JournalBulletin of the Korean Chemical Society
Volume38
Issue number4
DOIs
Publication statusPublished - 2017 Apr 1

Keywords

  • Nuclear magnetic resonance
  • RNA recognition motifs
  • Splicing
  • Squamous cell carcinoma antigen recognized by T-cells 3
  • Structure

ASJC Scopus subject areas

  • Chemistry(all)

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    Bang, K. M., Cho, N. Y., Kim, W. J., Kim, A. R., Song, H. K., Kim, E. E. K., & Kim, N. K. (2017). Structural Characterization of RNA Recognition Motif-2 Domain of SART3. Bulletin of the Korean Chemical Society, 38(4), 444-447. https://doi.org/10.1002/bkcs.11106