Structural consequences of aglycosylated IgG Fc variants evolved for FcγRI binding

Man Seok Ju, Jung Hyun Na, Yeon Gyu Yu, Jae Yeol Kim, Cherlhyun Jeong, Sang Taek Jung

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In contrast to the glycosylated IgG antibodies secreted by human plasma cells, the aglycosylated IgG antibodies produced by bacteria are unable to bind FcγRs expressed on the surface of immune effector cells and cannot trigger immune effector functions. To avoid glycan heterogeneity problems, elicit novel effector functions, and produce therapeutic antibodies with effector function using a simple bacterial expression system, FcγRI-specific Fc-engineered aglycosylated antibodies, Fc11 (E382V) and Fc (E382V/M428I), containing mutations in the CH3 region, were isolated in a previous study. To elucidate the relationship between FcγRI binding affinity and the structural dynamics of the upper CH2 region of Fc induced by the CH3 mutations, the conformational variation of Fc variants was observed by single-molecule Förster resonance energy transfer (FRET) analysis using alternating-laser excitation (ALEX). In sharp contrast to wild-type Fc, which exhibits a highly dynamic upper CH2 region, the mutations in the CH3 region significantly stabilized the upper CH2 region. The results indicate that conformational plasticity, as well as the openness of the upper CH2 region, is critical for FcγR binding and therapeutic effector functions of IgG antibodies.

Original languageEnglish
Pages (from-to)350-356
Number of pages7
JournalMolecular Immunology
Volume67
Issue number2
DOIs
Publication statusPublished - 2015 Oct 1
Externally publishedYes

Keywords

  • Aglycosylated antibody
  • Alternative laser excitation
  • Antibody engineering
  • Effector function
  • Förster resonance energy transfer
  • Single-molecule analysis

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

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  • Cite this

    Ju, M. S., Na, J. H., Yu, Y. G., Kim, J. Y., Jeong, C., & Jung, S. T. (2015). Structural consequences of aglycosylated IgG Fc variants evolved for FcγRI binding. Molecular Immunology, 67(2), 350-356. https://doi.org/10.1016/j.molimm.2015.06.020