Structural features of immunostimulatory polysaccharide purified from pectinase hydrolysate of barley leaf

Hoon Kim, Bong Shin Kwak, Hee Do Hong, Hyung Joo Suh, Kwang Soon Shin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Four polysaccharide fractions were isolated from young barley leaves treated with or without pectinase followed by ethanol fractionation. Among the polysaccharide fractions, BLE-P isolated from pectinase digested with a high molecular weight had the most enhanced macrophage stimulatory activity, indicating that pectinase digestion of barley leaf is a useful method for enhancement of its activity. BLE-P was further purified by column chromatography to identify the chemical and structural properties. BLE-P-I eluted in void volume fraction showed potent macrophage stimulatory activity. Monosaccharide composition and linkage analysis indicated that at least three kinds of polysaccharide, that is, glucuronoarabinoxylan (GAX; 40-45%), rhamnogalacturonan-I (RG-I) with branching mainly involving a type II arabinogalactan (AG-II) side chain (30-35%), and linear glucan such as starch and cellulose (less than 10%) coexisted in BLE-P-I. Given the association with macrophage stimulatory activity, it is likely that the GAX and to the RG-I polysaccharide branched with an AG-II side chain may be important for expression of the activity in barley leaf.

Original languageEnglish
Pages (from-to)308-316
Number of pages9
JournalInternational Journal of Biological Macromolecules
Volume87
DOIs
Publication statusPublished - 2016 Jun 1

Keywords

  • Barley leaf
  • Immunostimulatory activity
  • Pectinase digestion

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology

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