Structural features of immunostimulatory polysaccharide purified from pectinase hydrolysate of barley leaf

Hoon Kim, Bong Shin Kwak, Hee Do Hong, Hyung Joo Suh, Kwang Soon Shin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Four polysaccharide fractions were isolated from young barley leaves treated with or without pectinase followed by ethanol fractionation. Among the polysaccharide fractions, BLE-P isolated from pectinase digested with a high molecular weight had the most enhanced macrophage stimulatory activity, indicating that pectinase digestion of barley leaf is a useful method for enhancement of its activity. BLE-P was further purified by column chromatography to identify the chemical and structural properties. BLE-P-I eluted in void volume fraction showed potent macrophage stimulatory activity. Monosaccharide composition and linkage analysis indicated that at least three kinds of polysaccharide, that is, glucuronoarabinoxylan (GAX; 40-45%), rhamnogalacturonan-I (RG-I) with branching mainly involving a type II arabinogalactan (AG-II) side chain (30-35%), and linear glucan such as starch and cellulose (less than 10%) coexisted in BLE-P-I. Given the association with macrophage stimulatory activity, it is likely that the GAX and to the RG-I polysaccharide branched with an AG-II side chain may be important for expression of the activity in barley leaf.

Original languageEnglish
Pages (from-to)308-316
Number of pages9
JournalInternational Journal of Biological Macromolecules
Volume87
DOIs
Publication statusPublished - 2016 Jun 1

Fingerprint

Polygalacturonase
Hordeum
Polysaccharides
Macrophages
Column chromatography
Glucans
Monosaccharides
Fractionation
Cellulose
Starch
Chemical properties
Chromatography
Structural properties
Digestion
Volume fraction
Ethanol
Molecular Weight
Molecular weight
Association reactions
Chemical analysis

Keywords

  • Barley leaf
  • Immunostimulatory activity
  • Pectinase digestion

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Cite this

Structural features of immunostimulatory polysaccharide purified from pectinase hydrolysate of barley leaf. / Kim, Hoon; Kwak, Bong Shin; Hong, Hee Do; Suh, Hyung Joo; Shin, Kwang Soon.

In: International Journal of Biological Macromolecules, Vol. 87, 01.06.2016, p. 308-316.

Research output: Contribution to journalArticle

@article{d0baa3d6a53f47309e40d9db6bf09c37,
title = "Structural features of immunostimulatory polysaccharide purified from pectinase hydrolysate of barley leaf",
abstract = "Four polysaccharide fractions were isolated from young barley leaves treated with or without pectinase followed by ethanol fractionation. Among the polysaccharide fractions, BLE-P isolated from pectinase digested with a high molecular weight had the most enhanced macrophage stimulatory activity, indicating that pectinase digestion of barley leaf is a useful method for enhancement of its activity. BLE-P was further purified by column chromatography to identify the chemical and structural properties. BLE-P-I eluted in void volume fraction showed potent macrophage stimulatory activity. Monosaccharide composition and linkage analysis indicated that at least three kinds of polysaccharide, that is, glucuronoarabinoxylan (GAX; 40-45{\%}), rhamnogalacturonan-I (RG-I) with branching mainly involving a type II arabinogalactan (AG-II) side chain (30-35{\%}), and linear glucan such as starch and cellulose (less than 10{\%}) coexisted in BLE-P-I. Given the association with macrophage stimulatory activity, it is likely that the GAX and to the RG-I polysaccharide branched with an AG-II side chain may be important for expression of the activity in barley leaf.",
keywords = "Barley leaf, Immunostimulatory activity, Pectinase digestion",
author = "Hoon Kim and Kwak, {Bong Shin} and Hong, {Hee Do} and Suh, {Hyung Joo} and Shin, {Kwang Soon}",
year = "2016",
month = "6",
day = "1",
doi = "10.1016/j.ijbiomac.2016.02.072",
language = "English",
volume = "87",
pages = "308--316",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",

}

TY - JOUR

T1 - Structural features of immunostimulatory polysaccharide purified from pectinase hydrolysate of barley leaf

AU - Kim, Hoon

AU - Kwak, Bong Shin

AU - Hong, Hee Do

AU - Suh, Hyung Joo

AU - Shin, Kwang Soon

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Four polysaccharide fractions were isolated from young barley leaves treated with or without pectinase followed by ethanol fractionation. Among the polysaccharide fractions, BLE-P isolated from pectinase digested with a high molecular weight had the most enhanced macrophage stimulatory activity, indicating that pectinase digestion of barley leaf is a useful method for enhancement of its activity. BLE-P was further purified by column chromatography to identify the chemical and structural properties. BLE-P-I eluted in void volume fraction showed potent macrophage stimulatory activity. Monosaccharide composition and linkage analysis indicated that at least three kinds of polysaccharide, that is, glucuronoarabinoxylan (GAX; 40-45%), rhamnogalacturonan-I (RG-I) with branching mainly involving a type II arabinogalactan (AG-II) side chain (30-35%), and linear glucan such as starch and cellulose (less than 10%) coexisted in BLE-P-I. Given the association with macrophage stimulatory activity, it is likely that the GAX and to the RG-I polysaccharide branched with an AG-II side chain may be important for expression of the activity in barley leaf.

AB - Four polysaccharide fractions were isolated from young barley leaves treated with or without pectinase followed by ethanol fractionation. Among the polysaccharide fractions, BLE-P isolated from pectinase digested with a high molecular weight had the most enhanced macrophage stimulatory activity, indicating that pectinase digestion of barley leaf is a useful method for enhancement of its activity. BLE-P was further purified by column chromatography to identify the chemical and structural properties. BLE-P-I eluted in void volume fraction showed potent macrophage stimulatory activity. Monosaccharide composition and linkage analysis indicated that at least three kinds of polysaccharide, that is, glucuronoarabinoxylan (GAX; 40-45%), rhamnogalacturonan-I (RG-I) with branching mainly involving a type II arabinogalactan (AG-II) side chain (30-35%), and linear glucan such as starch and cellulose (less than 10%) coexisted in BLE-P-I. Given the association with macrophage stimulatory activity, it is likely that the GAX and to the RG-I polysaccharide branched with an AG-II side chain may be important for expression of the activity in barley leaf.

KW - Barley leaf

KW - Immunostimulatory activity

KW - Pectinase digestion

UR - http://www.scopus.com/inward/record.url?scp=84959484176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959484176&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2016.02.072

DO - 10.1016/j.ijbiomac.2016.02.072

M3 - Article

C2 - 26944661

AN - SCOPUS:84959484176

VL - 87

SP - 308

EP - 316

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -