Structural flexibility in the Burkholderia mallei genome

William C. Nierman, David DeShazer, H. Stanley Kim, Herve Tettelin, Karen E. Nelson, Tamara Feldblyum, Ricky L. Ulrich, Catherine M. Ronning, Lauren M. Brinkac, Sean C. Daugherty, Tanja D. Davidsen, Robert T. Deboy, George Dimitrov, Robert J. Dodson, A. Scott Durkin, Michelle L. Gwinn, Daniel H. Haft, Hoda Khouri, James F. Kolonay, Ramana MadupuYasmin Mohammoud, William C. Nelson, Diana Radune, Claudia M. Romero, Saul Sarria, Jeremy Selengut, Christine Shamblin, Steven A. Sullivan, Owen White, Yan Yu, Nikhat Zafar, Liwei Zhou, Claire M. Fraser

Research output: Contribution to journalArticle

254 Citations (Scopus)

Abstract

The complete genome sequence of Burkholderia mallei ATCC 23344 provides insight into this highly infectious bacterium's pathogenicity and evolutionary history. B. mallei, the etiologic agent of glanders, has come under renewed scientific investigation as a result of recent concerns about its past and potential future use as a biological weapon. Genome analysis identified a number of putative virulence factors whose function was supported by comparative genome hybridization and expression profiling of the bacterium in hamster liver in vivo. The genome contains numerous insertion sequence elements that have mediated extensive deletions and rearrangements of the genome relative to Burkholderia pseudomallei. The genome also contains a vast number (> 12,000) of simple sequence repeats. Variation in simple sequence repeats in key genes can provide a mechanism for generating antigenic variation that may account for the mammalian host's inability to mount a durable adaptive immune response to a B. mallei infection.

Original languageEnglish
Pages (from-to)14246-14251
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number39
DOIs
Publication statusPublished - 2004 Sep 28

ASJC Scopus subject areas

  • General

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    Nierman, W. C., DeShazer, D., Kim, H. S., Tettelin, H., Nelson, K. E., Feldblyum, T., Ulrich, R. L., Ronning, C. M., Brinkac, L. M., Daugherty, S. C., Davidsen, T. D., Deboy, R. T., Dimitrov, G., Dodson, R. J., Durkin, A. S., Gwinn, M. L., Haft, D. H., Khouri, H., Kolonay, J. F., ... Fraser, C. M. (2004). Structural flexibility in the Burkholderia mallei genome. Proceedings of the National Academy of Sciences of the United States of America, 101(39), 14246-14251. https://doi.org/10.1073/pnas.0403306101