Structural insights of the MenD from Escherichia coli reveal ThDP affinity

Amit Priyadarshi, Yasar Saleem, Ki Hyun Nam, Key Sun Kim, Sam Yong Park, Eunice EunKyeong Kim, Kwang Yeon Hwang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

MenD (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexadiene-1-carboxylate) synthase belongs to the superfamily of thiamin diphosphate-dependent decarboxylases, which converts isochorismate and 2-oxoglutarate to SHCHC, pyruvate, and carbon dioxide. Here, we report the first crystal structure of apo-MenD from Escherichia coli determined in tetragonal crystal form. The subunit displays the typical three-domain structure observed for ThDP-dependent enzymes. Analytical gel filtration shows that EcMenD behaves as a dimer as well as a tetramer. Circular dichroism and isothermal calorimetry results confirm EcMenD dependency on ThDP, which concomitantly helps to stabilize with better configuration.

Original languageEnglish
Pages (from-to)797-801
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number4
DOIs
Publication statusPublished - 2009 Mar 20

Fingerprint

Thiamine Pyrophosphate
Calorimetry
Carboxy-Lyases
Circular Dichroism
Pyruvic Acid
Carbon Dioxide
Dimers
Escherichia coli
Gel Chromatography
Crystal structure
Gels
Display devices
Crystals
Enzymes
1,4-cyclohexadiene
2-succinyl-6-hydroxycyclohexa-2,4-diene-1-carboxylic acid
alpha-ketoglutaric acid
isochorismic acid

Keywords

  • Decarboxylase
  • Menaquinone
  • MenD
  • Oxoglutarate
  • ThDP
  • Transferase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Structural insights of the MenD from Escherichia coli reveal ThDP affinity. / Priyadarshi, Amit; Saleem, Yasar; Nam, Ki Hyun; Kim, Key Sun; Park, Sam Yong; Kim, Eunice EunKyeong; Hwang, Kwang Yeon.

In: Biochemical and Biophysical Research Communications, Vol. 380, No. 4, 20.03.2009, p. 797-801.

Research output: Contribution to journalArticle

Priyadarshi, Amit ; Saleem, Yasar ; Nam, Ki Hyun ; Kim, Key Sun ; Park, Sam Yong ; Kim, Eunice EunKyeong ; Hwang, Kwang Yeon. / Structural insights of the MenD from Escherichia coli reveal ThDP affinity. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 380, No. 4. pp. 797-801.
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