Structural modification of siRNA for efficient gene silencing

So Jin Lee, Sejin Son, Ji Young Yhee, Kuiwon Choi, Ick Chan Kwon, Sun Hwa Kim, Kwang Meyung Kim

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Small interfering RNA (siRNA) holds a great promise for the future of genomic medicine because of its highly sequence-specific gene silencing and universality in therapeutic target. The medical use of siRNA, however, has been severely hampered by the inherent physico-chemical properties of siRNA itself, such as low charge density, high structural stiffness and rapid enzymatic degradation; therefore, the establishment of efficient and safe siRNA delivery methodology is an essential prerequisite, particularly for systemic administration. For an efficient systemic siRNA delivery, it is a critical issue to obtain small and compact siRNA polyplexes with cationic condensing reagents including cationic polymers, because the size and surface properties of the polyplexes are major determinants for achieving desirable in vivo fate. Unfortunately, synthetic siRNA is not easily condensed with cationic polymers due to its intrinsic rigid structure and low spatial charge density. Accordingly, the loose siRNA polyplexes inevitably expose siRNA to the extracellular environment during systemic circulation, resulting in low therapeutic efficiency and poor biodistribution. In this review, we highlight the innovative approaches to increase the size of siRNA via structural modification of the siRNA itself. The attempts include several methodologies such as hybridization, chemical polymerization, and micro- and nano-structurization of siRNA. Due to its increased charge density and flexibility, the structured siRNA can produce highly condensed and homogenous polyplexes compared to the classical monomeric siRNA. As a result, stable and compact siRNA polyplexes can enhance serum stability and target delivery efficiency in vivo with desirable biodistribution. The review specifically aims to provide the recent progress of structural modification of siRNA. In addition, the article also briefly and concisely explains the improved physico-chemical properties of structured siRNA with respect to stability, condensation ability and gene silencing efficiency.

Original languageEnglish
Pages (from-to)491-503
Number of pages13
JournalBiotechnology Advances
Volume31
Issue number5
DOIs
Publication statusPublished - 2013 Sep 1
Externally publishedYes

Fingerprint

Gene Silencing
Small Interfering RNA
Polymers
Surface Properties
Polymerization

Keywords

  • Polyplexes
  • RNAi
  • SiRNA
  • SiRNA delivery
  • Structured siRNA

ASJC Scopus subject areas

  • Biotechnology

Cite this

Structural modification of siRNA for efficient gene silencing. / Lee, So Jin; Son, Sejin; Yhee, Ji Young; Choi, Kuiwon; Kwon, Ick Chan; Kim, Sun Hwa; Kim, Kwang Meyung.

In: Biotechnology Advances, Vol. 31, No. 5, 01.09.2013, p. 491-503.

Research output: Contribution to journalArticle

Lee, SJ, Son, S, Yhee, JY, Choi, K, Kwon, IC, Kim, SH & Kim, KM 2013, 'Structural modification of siRNA for efficient gene silencing', Biotechnology Advances, vol. 31, no. 5, pp. 491-503. https://doi.org/10.1016/j.biotechadv.2012.09.002
Lee, So Jin ; Son, Sejin ; Yhee, Ji Young ; Choi, Kuiwon ; Kwon, Ick Chan ; Kim, Sun Hwa ; Kim, Kwang Meyung. / Structural modification of siRNA for efficient gene silencing. In: Biotechnology Advances. 2013 ; Vol. 31, No. 5. pp. 491-503.
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