Structural properties of cellulose and cellulase reaction mechanism

Sun Bok Lee, Ik Hwan Kim, Dewey D.Y. Ryu, H. Taguchi

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

The effects of structural properties and their changes during cellulose hydrolysis on the enzymatic hydrolysis rate have been studied from the reaction mechanism point of view. Important findings are the following: (1) The crystallinity index (CrI) of partially crystalline cellulose increases as the hydrolysis reaction proceeds, and a significant slowing down of the reaction rate during the enzymatic hydrolysis is, in large part, attributable to this structural change of cellulose substrate. (2) The crystallinity of completely disordered cellulose, like phosphoric‐acid‐treated cellulose, does not change significantly, and a relatively high hydrolysis rate is maintained during hydrolysis. (3) The specific surface area (SSA) of partially crystalline cellulose decreases significantly during enzymatic hydrolysis while the change in SSA of regenerated cellulose is found to be negligible. (4) The value of degree of polymerization (DP) of highly ordered crystalline cellulose remains practically constant whereas the change in DP of disordered regenerated cellulose is found to be very significant. (5) Combination of these structural effects as well as cellulase adsorption, product inhibition, and cellulase deactivation all have important influence on the rate of cellulase reaction during cellulose hydrolysis. More experimental evidence for a two‐phase model, which is based on degradation of cellulose by simultaneous actions of cellulase complex on the crystalline and amorphous phases, has been obtained. Based on experimental results from this study and other results accumulated, the mode of cellulase action and a possible reaction mechanism are proposed.

Original languageEnglish
Pages (from-to)33-51
Number of pages19
JournalBiotechnology and Bioengineering
Volume25
Issue number1
DOIs
Publication statusPublished - 1983
Externally publishedYes

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Cellulase
Cellulose
Structural properties
Hydrolysis
Enzymatic hydrolysis
Crystalline materials
Specific surface area
Polymerization
Adsorption
Reaction rates

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Structural properties of cellulose and cellulase reaction mechanism. / Lee, Sun Bok; Kim, Ik Hwan; Ryu, Dewey D.Y.; Taguchi, H.

In: Biotechnology and Bioengineering, Vol. 25, No. 1, 1983, p. 33-51.

Research output: Contribution to journalArticle

Lee, Sun Bok ; Kim, Ik Hwan ; Ryu, Dewey D.Y. ; Taguchi, H. / Structural properties of cellulose and cellulase reaction mechanism. In: Biotechnology and Bioengineering. 1983 ; Vol. 25, No. 1. pp. 33-51.
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