Structural Studies of a Bacterial Condensin Complex Reveal ATP-Dependent Disruption of Intersubunit Interactions

Jae Sung Woo, Jae Hong Lim, Ho Chul Shin, Min Kang Suh, Bonsu Ku, Kwang Hoon Lee, Keehyoung Joo, Howard Robinson, Jooyoung Lee, Sam Yong Park, Nam Chul Ha, Byung Ha Oh

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Condensins are key mediators of chromosome condensation across organisms. Like other condensins, the bacterial MukBEF condensin complex consists of an SMC family protein dimer containing two ATPase head domains, MukB, and two interacting subunits, MukE and MukF. We report complete structural views of the intersubunit interactions of this condensin along with ensuing studies that reveal a role for the ATPase activity of MukB. MukE and MukF together form an elongated dimeric frame, and MukF's C-terminal winged-helix domains (C-WHDs) bind MukB heads to constitute closed ring-like structures. Surprisingly, one of the two bound C-WHDs is forced to detach upon ATP-mediated engagement of MukB heads. This detachment reaction depends on the linker segment preceding the C-WHD, and mutations on the linker restrict cell growth. Thus ATP-dependent transient disruption of the MukB-MukF interaction, which creates openings in condensin ring structures, is likely to be a critical feature of the functional mechanism of condensins.

Original languageEnglish
Pages (from-to)85-96
Number of pages12
JournalCell
Volume136
Issue number1
DOIs
Publication statusPublished - 2009 Jan 9
Externally publishedYes

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Adenosine Triphosphate
Adenosine Triphosphatases
Head
Cell growth
Chromosomes
Dimers
condensin complexes
Condensation
Mutation
Growth
Proteins

Keywords

  • CELLBIO
  • PROTEINS

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Structural Studies of a Bacterial Condensin Complex Reveal ATP-Dependent Disruption of Intersubunit Interactions. / Woo, Jae Sung; Lim, Jae Hong; Shin, Ho Chul; Suh, Min Kang; Ku, Bonsu; Lee, Kwang Hoon; Joo, Keehyoung; Robinson, Howard; Lee, Jooyoung; Park, Sam Yong; Ha, Nam Chul; Oh, Byung Ha.

In: Cell, Vol. 136, No. 1, 09.01.2009, p. 85-96.

Research output: Contribution to journalArticle

Woo, JS, Lim, JH, Shin, HC, Suh, MK, Ku, B, Lee, KH, Joo, K, Robinson, H, Lee, J, Park, SY, Ha, NC & Oh, BH 2009, 'Structural Studies of a Bacterial Condensin Complex Reveal ATP-Dependent Disruption of Intersubunit Interactions', Cell, vol. 136, no. 1, pp. 85-96. https://doi.org/10.1016/j.cell.2008.10.050
Woo, Jae Sung ; Lim, Jae Hong ; Shin, Ho Chul ; Suh, Min Kang ; Ku, Bonsu ; Lee, Kwang Hoon ; Joo, Keehyoung ; Robinson, Howard ; Lee, Jooyoung ; Park, Sam Yong ; Ha, Nam Chul ; Oh, Byung Ha. / Structural Studies of a Bacterial Condensin Complex Reveal ATP-Dependent Disruption of Intersubunit Interactions. In: Cell. 2009 ; Vol. 136, No. 1. pp. 85-96.
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