Structure and expression of the orphan nuclear receptor SHP gene

Hye Kyung Lee, Yoon Kwang Lee, Sun-Hwa Park, Youn Sook Kim, Seong Hoe Park, Jae Woon Lee, Hyuk Bang Kwon, Jaemog Soh, David D. Moore, Hueng Sik Choi

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

To determine the organization of the orphan nuclear receptor SHP gene (Seol, W., Choi, H.-S., and Moore, D.D. (1996) Science 272, 1336-1339), genomic clones were isolated from human and mouse genomic libraries. The SHP gene was composed of two exons interrupted by a single intron spanning approximately 1.8 kilobases in human and 1.2 kilobases in mouse. Genomic Southern blot analysis and fluorescence in situ hybridization of human metaphase chromosomes indicated that the SlIP gene is located at the human chromosome 1p36.1 sub-band. The 5'-flanking regions of human and mouse SHP genes were highly conserved, showing 77% homology in the region of approximately 600 nucleotides upstream from the transcription start site. Primer extension analysis was carried out to determine the transcription start site of human SHP to 32 nucleotides downstream of a potential TATA box. The human SHP gene was specifically expressed in fetal liver, fetal adrenal gland, adult spleen, and adult small intestine. As expected from this expression pattern, the activity of the mouse SHP promoter measured by transient transfection was significantly higher in the adrenal-derived Y1 cells than HeLa cells.

Original languageEnglish
Pages (from-to)14398-14402
Number of pages5
JournalJournal of Biological Chemistry
Volume273
Issue number23
DOIs
Publication statusPublished - 1998 Jun 5

Fingerprint

Genes
Transcription Initiation Site
Human Chromosomes
Chromosomes
Nucleotides
TATA Box
Genomic Library
5' Flanking Region
Metaphase
Adrenal Glands
Southern Blotting
Fluorescence In Situ Hybridization
HeLa Cells
Liver
Introns
Small Intestine
Transfection
Exons
Spleen
Clone Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lee, H. K., Lee, Y. K., Park, S-H., Kim, Y. S., Park, S. H., Lee, J. W., ... Choi, H. S. (1998). Structure and expression of the orphan nuclear receptor SHP gene. Journal of Biological Chemistry, 273(23), 14398-14402. https://doi.org/10.1074/jbc.273.23.14398

Structure and expression of the orphan nuclear receptor SHP gene. / Lee, Hye Kyung; Lee, Yoon Kwang; Park, Sun-Hwa; Kim, Youn Sook; Park, Seong Hoe; Lee, Jae Woon; Kwon, Hyuk Bang; Soh, Jaemog; Moore, David D.; Choi, Hueng Sik.

In: Journal of Biological Chemistry, Vol. 273, No. 23, 05.06.1998, p. 14398-14402.

Research output: Contribution to journalArticle

Lee, HK, Lee, YK, Park, S-H, Kim, YS, Park, SH, Lee, JW, Kwon, HB, Soh, J, Moore, DD & Choi, HS 1998, 'Structure and expression of the orphan nuclear receptor SHP gene', Journal of Biological Chemistry, vol. 273, no. 23, pp. 14398-14402. https://doi.org/10.1074/jbc.273.23.14398
Lee, Hye Kyung ; Lee, Yoon Kwang ; Park, Sun-Hwa ; Kim, Youn Sook ; Park, Seong Hoe ; Lee, Jae Woon ; Kwon, Hyuk Bang ; Soh, Jaemog ; Moore, David D. ; Choi, Hueng Sik. / Structure and expression of the orphan nuclear receptor SHP gene. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 23. pp. 14398-14402.
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