Structure-based studies on the metal binding of two-metal-dependent sugar isomerases

Ponnandy Prabhu, Thi Ngoc Thanh Doan, Manish Tiwari, Raushan Singh, Sun Chang Kim, Myoung Ki Hong, Yun Chan Kang, Lin Woo Kang, Jung Kul Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Two-metal-dependent sugar isomerases are important in the synthesis of rare sugars. Many of their properties, specifically their metal dependency, have not been sufficiently explored. Here we used X-ray crystallography, site-directed mutagenesis, isothermal titration calorimetry and electron paramagnetic resonance spectroscopy to investigate the molecular determinants of the metal-binding affinity of l-rhamnose isomerase, a two-Mn 2+ -dependent isomerase from Bacillus halodurans (BHRI). The crystal structure of BHRI confirmed the presence of two metal ion-binding sites: a structural metal ion-binding site for substrate binding, and a catalytic metal ion-binding site that catalyzes a hydride shift. One conserved amino acid, W38, in wild-type BHRI was identified as a critical residue for structural Mn 2+ binding and thus the catalytic efficiency of BHRI. This function of W38 was explored by replacing it with other amino acids. Substitution by Phe, His, Lys, Ile or Ala caused complete loss of catalytic activity. The role of W38 was further examined by analyzing the crystal structure of wild-type BHRI and two inactive mutants of BHRI (W38F and W38A) in complex with Mn 2+ . A structural comparison of the mutants and the wild-type revealed differences in their coordination of Mn 2+ , including changes in metal-ligand bond length and affinity for Mn 2+ . The role of W38 was further confirmed in another two-metal-dependent enzyme: xylose isomerase from Bacillus licheniformis. These data suggest that W38 stabilizes protein-metal complexes and in turn assists ligand binding during catalysis in two-metal-dependent isomerases.

Original languageEnglish
Pages (from-to)3446-3459
Number of pages14
JournalFEBS Journal
Volume281
Issue number15
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Fingerprint

Isomerases
Sugars
Metals
Metal ions
Binding Sites
Bacilli
xylose isomerase
Crystal structure
Structural metals
Ions
Ligands
Amino Acids
Rhamnose
Mutagenesis
X ray crystallography
Coordination Complexes
Bond length
Calorimetry
Titration
Hydrides

Keywords

  • Bacillus halodurans
  • crystal structure
  • isomerase
  • molecular determinant
  • rare sugar

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Prabhu, P., Doan, T. N. T., Tiwari, M., Singh, R., Kim, S. C., Hong, M. K., ... Lee, J. K. (2014). Structure-based studies on the metal binding of two-metal-dependent sugar isomerases. FEBS Journal, 281(15), 3446-3459. https://doi.org/10.1111/febs.12872

Structure-based studies on the metal binding of two-metal-dependent sugar isomerases. / Prabhu, Ponnandy; Doan, Thi Ngoc Thanh; Tiwari, Manish; Singh, Raushan; Kim, Sun Chang; Hong, Myoung Ki; Kang, Yun Chan; Kang, Lin Woo; Lee, Jung Kul.

In: FEBS Journal, Vol. 281, No. 15, 01.01.2014, p. 3446-3459.

Research output: Contribution to journalArticle

Prabhu, P, Doan, TNT, Tiwari, M, Singh, R, Kim, SC, Hong, MK, Kang, YC, Kang, LW & Lee, JK 2014, 'Structure-based studies on the metal binding of two-metal-dependent sugar isomerases', FEBS Journal, vol. 281, no. 15, pp. 3446-3459. https://doi.org/10.1111/febs.12872
Prabhu P, Doan TNT, Tiwari M, Singh R, Kim SC, Hong MK et al. Structure-based studies on the metal binding of two-metal-dependent sugar isomerases. FEBS Journal. 2014 Jan 1;281(15):3446-3459. https://doi.org/10.1111/febs.12872
Prabhu, Ponnandy ; Doan, Thi Ngoc Thanh ; Tiwari, Manish ; Singh, Raushan ; Kim, Sun Chang ; Hong, Myoung Ki ; Kang, Yun Chan ; Kang, Lin Woo ; Lee, Jung Kul. / Structure-based studies on the metal binding of two-metal-dependent sugar isomerases. In: FEBS Journal. 2014 ; Vol. 281, No. 15. pp. 3446-3459.
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