Structure of the autophagic E2 enzyme Atg10

Seung Beom Hong, Byeong Won Kim, Jun Hoe Kim, Hyun Kyu Song

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Autophagy is a regulated degradation pathway that plays a critical role in all eukaryotic life cycles. One interesting feature of the core autophagic process, autophagosome formation, is similar to ubiquitination. One of two autophagic E2 enzymes, Atg10, interacts with Atg7 to receive Atg12, a ubiquitin-like molecule, and is also involved in the Atg12-Atg5 conjugation reaction. To date, no information on the interaction between Atg10 and Atg7 has been reported, although structural information is available pertaining to the individual components. Here, the crystal structure of Atg10 from Saccharo-myces cerevisiae is described at 2.7 Å resolution. A significant improvement of the diffraction limit by heavy-atom deriv-atization was essential for structure determination. The core fold of yeast Atg10 is well conserved compared with those of Atg3 and other E2 enzymes. In contrast to other E2 enzymes, however, the autophagic E2 enzymes Atg3 and Atg10 possess insertion regions in the middle of the core fold and may be involved in protein function. The missing segment, which was termed the FR-region, in Atg10 may be important for interaction with the E1 enzyme Atg7. This study provides a framework for understanding the E2 conjugation reaction in autophagy.

Original languageEnglish
Pages (from-to)1409-1417
Number of pages9
JournalActa Crystallographica Section D: Biological Crystallography
Volume68
Issue number10
DOIs
Publication statusPublished - 2012 Oct 1

Fingerprint

Enzymes
Autophagy
Ubiquitination
Ubiquitin
Life Cycle Stages
Yeasts
Proteins
Autophagosomes

Keywords

  • Atg10
  • Atg12
  • Atg3
  • Atg5
  • Atg7
  • autophagy
  • E2 enzymes
  • Saccharomyces cerevisiae
  • ubiquitination

ASJC Scopus subject areas

  • Structural Biology

Cite this

Structure of the autophagic E2 enzyme Atg10. / Hong, Seung Beom; Kim, Byeong Won; Kim, Jun Hoe; Song, Hyun Kyu.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 68, No. 10, 01.10.2012, p. 1409-1417.

Research output: Contribution to journalArticle

Hong, Seung Beom ; Kim, Byeong Won ; Kim, Jun Hoe ; Song, Hyun Kyu. / Structure of the autophagic E2 enzyme Atg10. In: Acta Crystallographica Section D: Biological Crystallography. 2012 ; Vol. 68, No. 10. pp. 1409-1417.
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