Studies on the distribution of O6-methylguanine-dna methyltransferase in rat

Jun Gil-Ja Jun, Ro Jai-Youl Ro, Hee Kim Myung Hee Kim, Gil-Hong Park, Ki Paik Woon Ki Paik, Peter N. Magee, Kim Sangduk

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

O6-Methylguanine-DNA methyltransferase, a DNA repair enzyme which transfers the methyl group of O6-methylguanine residue to a cysteinyl residue in the methyltransferase itself, was examined in rat organs by quantifying the S-methylcysteine formed in the methyl acceptor protein. Among the various organs examined, the spleen exhibited the highest enzyme specific activity followed by the thymus, liver, lung and testis. Brain had the lowest activity. The patterns of subcellular distribution of the methyltransferase in spleen and liver were different: while 75-80% of the activity was present in the nuclear fraction of the spleen, 54% of the activity in the liver was found in the nuclei and 35% in the cytosolic fraction. Forty-five and thirty-five percent of the total nuclear enzyme activity could be extracted with 1 M and 2 M NaCl solutions, respectively, indicating that the repair enzyme is not tightly bound to the nuclear matrix. When isolated nuclei were incubated with [methyl-3H]DNA substrate and subsequently fractionated into histone and non-histone protein fractions, over 90% of the radioactivity was coeluted on a Bio-Rex 70 column with the non-histone fraction and only a negligible amount of radioactivity was found to be associated with the histone fraction. The molecular mass of the [methyl-3H]methyltransferase in the non-histone fraction was determined to be 23,000, and its pI value was found to be 6.6 by two-dimensional polyacrylamide gel electrophoresis.

Original languageEnglish
Pages (from-to)377-384
Number of pages8
JournalBiochemical Pharmacology
Volume35
Issue number3
DOIs
Publication statusPublished - 1986 Feb 1
Externally publishedYes

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Methyltransferases
Rats
Liver
Spleen
Radioactivity
Histones
Enzymes
DNA Repair Enzymes
Nuclear Matrix
Thymus
DNA
Electrophoresis, Gel, Two-Dimensional
Enzyme activity
Molecular mass
Electrophoresis
Thymus Gland
Testis
Brain
Proteins
Repair

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

Cite this

Gil-Ja Jun, J., Jai-Youl Ro, R., Myung Hee Kim, H. K., Park, G-H., Woon Ki Paik, K. P., Magee, P. N., & Sangduk, K. (1986). Studies on the distribution of O6-methylguanine-dna methyltransferase in rat. Biochemical Pharmacology, 35(3), 377-384. https://doi.org/10.1016/0006-2952(86)90208-X

Studies on the distribution of O6-methylguanine-dna methyltransferase in rat. / Gil-Ja Jun, Jun; Jai-Youl Ro, Ro; Myung Hee Kim, Hee Kim; Park, Gil-Hong; Woon Ki Paik, Ki Paik; Magee, Peter N.; Sangduk, Kim.

In: Biochemical Pharmacology, Vol. 35, No. 3, 01.02.1986, p. 377-384.

Research output: Contribution to journalArticle

Gil-Ja Jun, J, Jai-Youl Ro, R, Myung Hee Kim, HK, Park, G-H, Woon Ki Paik, KP, Magee, PN & Sangduk, K 1986, 'Studies on the distribution of O6-methylguanine-dna methyltransferase in rat', Biochemical Pharmacology, vol. 35, no. 3, pp. 377-384. https://doi.org/10.1016/0006-2952(86)90208-X
Gil-Ja Jun J, Jai-Youl Ro R, Myung Hee Kim HK, Park G-H, Woon Ki Paik KP, Magee PN et al. Studies on the distribution of O6-methylguanine-dna methyltransferase in rat. Biochemical Pharmacology. 1986 Feb 1;35(3):377-384. https://doi.org/10.1016/0006-2952(86)90208-X
Gil-Ja Jun, Jun ; Jai-Youl Ro, Ro ; Myung Hee Kim, Hee Kim ; Park, Gil-Hong ; Woon Ki Paik, Ki Paik ; Magee, Peter N. ; Sangduk, Kim. / Studies on the distribution of O6-methylguanine-dna methyltransferase in rat. In: Biochemical Pharmacology. 1986 ; Vol. 35, No. 3. pp. 377-384.
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