Studies on the production of cellulase by Trichoderma sp. SO-571 and the enzyme treatment for cellulosic fabrics

Sung Hoon Oh, Moo Sung Kim, Sung So, Hyung Joo Suh

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Abstract

A Trichoderma sp. SO-571 producing cellulase was isolated from soil, and a pilot-scale cultivation and separation of cellulase were conducted. The cellulase activity was about 14.5 unit/ml after 112 hr of cultivation in a 30 1 fermenter containing 3.0% cellulose, 4.0% soybean powder, 3.0% wheat bran, 0.5% (NH4)2SO4, 0.2% urea, 1.0% CSL, 0.5% KH 2PO4, and 0.2% Tween 80. The cellulase was purified over 4.6 folds in three steps with 47.86% yield. The optimum pH of cellulase was pH 5.0 and optimum temperature was 60°C. To investigate the effect of the cellulase-treated cellulosic fabric, the weight loss was compared. The weight loss of denim treated with cellulase from Trichoderma sp. SO-571 was 2.9% and that with Celluclast 1.5L was 2.2%. In tencel treatement with enzyme, cellulase showed 0.7% higer weight loss than that with Celluclast 1.5L.

Original languageEnglish
Pages (from-to)42-45
Number of pages4
JournalKorean Journal of Microbiology and Biotechnology
Volume31
Issue number1
Publication statusPublished - 2003 Mar 1

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Keywords

  • Cellulase
  • Denim
  • Tencel
  • Trichoderma sp.

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology

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