Study of Alzheimer’s disease-related biophysical kinetics with a microslit-embedded cantilever sensor in a liquid environment

Myung Sic Chae, Jinsik Kim, Yong Kyoung Yoo, Jeong Hoon Lee, Tae Geun Kim, Kyo Seon Hwang

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A microsized slit-embedded cantilever sensor (slit cantilever) was fabricated and evaluated as a biosensing platform in a liquid environment. In order to minimize the degradation caused by viscous damping, a 300 × 100 μm2 (length × width) sized cantilever was released by a 5 μm gap-surrounding and vibrated by an internal piezoelectric-driven self-actuator. Owing to the structure, when the single side of the slit cantilever was exposed to liquid a significant quality factor (Q = 35) could be achieved. To assess the sensing performance, the slit cantilever was exploited to study the biophysical kinetics related to Aβ peptide. First, the quantification of Aβ peptide with a concentration of 10 pg/mL to 1 βg/mL was performed. The resonant responses exhibited a dynamic range from 100 pg/mL to 100 ng/mL (-56.5 to -774 ΔHz) and a dissociation constant (KD) of binding affinity was calculated as 1.75 nM. Finally, the Aβ self-aggregation associated with AD pathogenesis was monitored by adding monomeric Aβ peptides. As the concentration of added analyte increased from 100 ng/mL to 10 μg/mL, both the frequency shift values (-813 to -1804 ΔHz) and associate time constant increased. These results showed the excellent sensing performance of the slit cantilever overcoming a major drawback in liquid environments to become a promising diagnostic tool candidate.

Original languageEnglish
Article number1819
JournalSensors (Switzerland)
Volume17
Issue number8
DOIs
Publication statusPublished - 2017 Aug 7

Keywords

  • Alzheimer’s disease
  • Biosensor
  • Cantilever sensor
  • Viscous damping

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Electrical and Electronic Engineering

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