Study of erythrocyte membrane fluctuation using light scattering analysis

Hoyoon Lee, Sangyun Lee, Yongkeun Park, Sehyun Shin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

It is commonly known that alteration of erythrocyte deformability lead to serious microcirculatory diseases such as retinopathy, nephropathy, etc. Various methods and technologies have been developed to diagnose such membrane properties of erythrocytes. In this study, we developed an innovative method to measure hemorheological characteristics of the erythrocyte membrane using a light scattering analysis with simplified optic setting and multi-cell analysis as well. Light scattering intensity through multiple erythrocytes and its power density spectrum were obtained. The results of light scattering analyses were compared in healthy control and artificially hardened sample which was treated with glutaraldehyde. These results were further compared with conventional assays to measure deformable property in hemorheology. We found that light scattering information would reflect the disturbance of membrane fluctuation in artificially damaged erythrocytes. Therefore, measuring fluctuation of erythrocyte membrane using light scattering signal could facilitate simple and precise diagnose of pathological state on erythrocyte as well as related complications.

Original languageEnglish
Title of host publicationQuantitative Phase Imaging II
PublisherSPIE
Volume9718
ISBN (Electronic)9781628419528
DOIs
Publication statusPublished - 2016
Event2nd Conference on Quantitative Phase Imaging, QPI 2016 - San Francisco, United States
Duration: 2016 Feb 142016 Feb 17

Other

Other2nd Conference on Quantitative Phase Imaging, QPI 2016
CountryUnited States
CitySan Francisco
Period16/2/1416/2/17

Keywords

  • Deformability
  • Erythrocyte
  • Light scattering

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Lee, H., Lee, S., Park, Y., & Shin, S. (2016). Study of erythrocyte membrane fluctuation using light scattering analysis. In Quantitative Phase Imaging II (Vol. 9718). [971818] SPIE. https://doi.org/10.1117/12.2210777