Study of hyperpigmentation in human skin disorder using different electron microscopy techniques

Seulgi Noh, Hyosun Choi, Ji Soo Kim, Il-Hwan Kim, Ji Young Mun

Research output: Contribution to journalArticle

Abstract

There is a global trend of increase in the demand for three-dimensional electron microscopy with high resolution. The ultrastructural change and related functional studies are necessary to investigate biological phenomena. In this study, currently available 3D reconstruction techniques of electron microscopes (serial block-face scanning electron microscopy and focused ion beam—scanning electron microscopy) were used to investigate hyperpigmentary disorders in human skin. In the basal layer of the epidermis in the human skin, there are melanocytes that produce melanin and keratinocytes that act as a barrier against environmental damage. The 3D structure from serial images through scanning electron microscopy showed locations of melanosomes between melanocyte and keratinocyte in the hyperpigmentary disorder, in addition, the electron tomography showed pigment transfer through melanin instead melanosome. These results support the exocytosis-endocytosis theory of pigment in human skin.

Original languageEnglish
Pages (from-to)18-24
Number of pages7
JournalMicroscopy Research and Technique
Volume82
Issue number1
DOIs
Publication statusPublished - 2019 Jan 1

Fingerprint

melanin
Hyperpigmentation
pigments
Melanin
Electron microscopy
Melanosomes
electron microscopy
Electron Microscopy
Skin
Melanocytes
Melanins
disorders
Keratinocytes
Pigments
epidermis
Electron Scanning Microscopy
scanning electron microscopy
Electron Microscope Tomography
Biological Phenomena
Scanning electron microscopy

Keywords

  • 3D electron microscopy
  • hyperpigmentation
  • keratinocyte
  • melanocyte
  • melasma
  • seborrheic keratosis

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

Cite this

Study of hyperpigmentation in human skin disorder using different electron microscopy techniques. / Noh, Seulgi; Choi, Hyosun; Kim, Ji Soo; Kim, Il-Hwan; Mun, Ji Young.

In: Microscopy Research and Technique, Vol. 82, No. 1, 01.01.2019, p. 18-24.

Research output: Contribution to journalArticle

Noh, Seulgi ; Choi, Hyosun ; Kim, Ji Soo ; Kim, Il-Hwan ; Mun, Ji Young. / Study of hyperpigmentation in human skin disorder using different electron microscopy techniques. In: Microscopy Research and Technique. 2019 ; Vol. 82, No. 1. pp. 18-24.
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