Study on chemotaxis and chemokinesis of bone marrow-derived mesenchymal stem cells in hydrogel-based 3D microfluidic devices

Dayoung Yoon, Hyerim Kim, Eojin Lee, Min Hee Park, Seok Chung, Hojeong Jeon, Cheol Hee Ahn, Kangwon Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Controlling the fate of mesenchymal stems cells (MSCs) including proliferation, migration and differentiation has recently been studied by many researchers in the tissue engineering field. Especially, recruitment of stem cells to injury sites is the first and crucial step in tissue regeneration. Although significant progress has been made in the chemotactic migration of MSCs, MSC migration in three dimensional environments remains largely unknown. We developed a 3D hydrogel-based microfluidic-device to study the migration behavior of human MSCs in the presence of stromal-cell derived factor-1α (SDF-1α), interleukin 8 (IL-8) and Substance P (SP) which have been utilized as chemoattractant candidates of human mesenchymal stem cells (hMSCs). Results: We systematically investigated the chemotactic migration behaviors of hMSCs and their responses to SDF-1a, IL-8, and SP. SDF-1α was shown to be the most fascinating chemoattractant candidate among those factors at a certain time point. We also found that each chemokine showed different chemoattractant abilities according to their concentration. In the case of SP, this factor showed chemokinesis not chemotaxis. Especially at a 7-8×10-8 M concentration range, the chemokinesis ability driven by SP was further increased. The data suggest that some factors at the optimal concentration exhibit chemokinesis or chemotaxis in a 3D hydrogel-based microfluidic device. Conclusion: In this study on chemotaxis and chemokinesis of hMSCs, the system parameters such as chemokine concentration, system stability, and 2D or 3D microenvironment are critically important to obtain meaningful results.

Original languageEnglish
Article number25
JournalBiomaterials Research
Volume20
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

Fingerprint

Lab-On-A-Chip Devices
Hydrogel
Chemotaxis
Stem cells
Mesenchymal Stromal Cells
Microfluidics
Hydrogels
Bone
Bone Marrow
Substance P
Chemotactic Factors
Chemokine CXCL12
Aptitude
Interleukin-8
Chemokines
Tissue regeneration
Tissue Engineering
Cell proliferation
Cell Movement
System stability

Keywords

  • Chemokinesis
  • Chemotaxis
  • Mesenchymal stem cells
  • Microfluidic device

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Medicine (miscellaneous)

Cite this

Study on chemotaxis and chemokinesis of bone marrow-derived mesenchymal stem cells in hydrogel-based 3D microfluidic devices. / Yoon, Dayoung; Kim, Hyerim; Lee, Eojin; Park, Min Hee; Chung, Seok; Jeon, Hojeong; Ahn, Cheol Hee; Lee, Kangwon.

In: Biomaterials Research, Vol. 20, No. 1, 25, 01.01.2016.

Research output: Contribution to journalArticle

Yoon, Dayoung ; Kim, Hyerim ; Lee, Eojin ; Park, Min Hee ; Chung, Seok ; Jeon, Hojeong ; Ahn, Cheol Hee ; Lee, Kangwon. / Study on chemotaxis and chemokinesis of bone marrow-derived mesenchymal stem cells in hydrogel-based 3D microfluidic devices. In: Biomaterials Research. 2016 ; Vol. 20, No. 1.
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