Subcellular progression of mesenchymal transition identified by two discrete synchronous cell lines derived from the same glioblastoma

Sojin Kim, Soo Ji Park, Tamrin Chowdhury, Jeong Im Hong, Jinhee Ahn, Tae Yeong Jeong, Hyeon Jong Yu, Young Kyoung Shin, Ja Lok Ku, Jong Bae Park, Junho K. Hur, Hwajin Lee, Kyoungmi Kim, Chul‑Kee ‑K Park

Research output: Contribution to journalArticlepeer-review

Abstract

Glioblastomas (GBM) exhibit intratumoral heterogeneity of various oncogenic evolutional processes. We have successfully isolated and established two distinct cancer cell lines with different morphological and biological characteristics that were derived from the same tissue sample of a GBM. When we compared their genomic and transcriptomic characteristics, each cell line harbored distinct mutation clusters while sharing core driver mutations. Transcriptomic analysis revealed that one cell line was undergoing a mesenchymal transition process, unlike the other cell line. Furthermore, we could identify four tumor samples containing our cell line-like clusters from the publicly available single-cell RNA-seq data, and in a set of paired longitudinal GBM samples, we could confirm three pairs where the recurrent sample was enriched in the genes specific to our cell line undergoing mesenchymal transition. The present study provides direct evidence and a valuable source for investigating the ongoing process of subcellular mesenchymal transition in GBM, which has prognostic and therapeutic implications.

Original languageEnglish
Article number181
JournalCellular and Molecular Life Sciences
Volume79
Issue number3
DOIs
Publication statusPublished - 2022 Mar

Keywords

  • Glioblastoma
  • Intratumoral heterogeneity
  • Mesenchymal transition
  • Synchronous cell lines

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

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