Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus

Byoung Shik Shim, Jung ah Choi, Ho Hyun Song, Sung Moo Park, In Su Cheon, Ji Eun Jang, Sun Je Woo, Chung Hwan Cho, Min Suk Song, Hyemi Kim, Kyung Joo Song, Jae Myun Lee, Suhng Wook Kim, Dae Sub Song, Young Ki Choi, Jae Ouk Kim, Huan Huu Nguyen, Dong Wook Kim, Young Yil Bahk, Cheol Heui YunMan Ki Song

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s. l.) route. We found that s. l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i. m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s. l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.

Original languageEnglish
Pages (from-to)130-135
Number of pages6
JournalJournal of Microbiology
Volume51
Issue number1
DOIs
Publication statusPublished - 2013 Feb

Keywords

  • Hemagglutinin
  • mucosal immune response
  • non-glycosylation
  • pandemic
  • sublingual

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus'. Together they form a unique fingerprint.

  • Cite this

    Shim, B. S., Choi, J. A., Song, H. H., Park, S. M., Cheon, I. S., Jang, J. E., Woo, S. J., Cho, C. H., Song, M. S., Kim, H., Song, K. J., Lee, J. M., Kim, S. W., Song, D. S., Choi, Y. K., Kim, J. O., Nguyen, H. H., Kim, D. W., Bahk, Y. Y., ... Song, M. K. (2013). Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus. Journal of Microbiology, 51(1), 130-135. https://doi.org/10.1007/s12275-013-2399-z