Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus

Byoung Shik Shim, Jung ah Choi, Ho Hyun Song, Sung Moo Park, In Su Cheon, Ji Eun Jang, Sun Je Woo, Chung Hwan Cho, Min Suk Song, Hyemi Kim, Kyung Joo Song, Jae Myun Lee, Suhng Wook Kim, Dae Sub Song, Young Ki Choi, Jae Ouk Kim, Huan Huu Nguyen, Dong Wook Kim, Young Yil Bahk, Cheol Heui YunMan Ki Song

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s. l.) route. We found that s. l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i. m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s. l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.

Original languageEnglish
Pages (from-to)130-135
Number of pages6
JournalJournal of Microbiology
Volume51
Issue number1
DOIs
Publication statusPublished - 2013 Feb 1

Fingerprint

Sublingual Administration
H1N1 Subtype Influenza A Virus
Pandemics
Orthomyxoviridae
Human Influenza
Bacteria
Infection
Disease Outbreaks
Immunization
Vaccination
Vaccines
Influenza Vaccines
Cholera Toxin
Influenza A virus
Neutralizing Antibodies
Proteins
Morbidity
Mortality
hemagglutinin I
Antibodies

Keywords

  • Hemagglutinin
  • mucosal immune response
  • non-glycosylation
  • pandemic
  • sublingual

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus. / Shim, Byoung Shik; Choi, Jung ah; Song, Ho Hyun; Park, Sung Moo; Cheon, In Su; Jang, Ji Eun; Woo, Sun Je; Cho, Chung Hwan; Song, Min Suk; Kim, Hyemi; Song, Kyung Joo; Lee, Jae Myun; Kim, Suhng Wook; Song, Dae Sub; Choi, Young Ki; Kim, Jae Ouk; Nguyen, Huan Huu; Kim, Dong Wook; Bahk, Young Yil; Yun, Cheol Heui; Song, Man Ki.

In: Journal of Microbiology, Vol. 51, No. 1, 01.02.2013, p. 130-135.

Research output: Contribution to journalArticle

Shim, BS, Choi, JA, Song, HH, Park, SM, Cheon, IS, Jang, JE, Woo, SJ, Cho, CH, Song, MS, Kim, H, Song, KJ, Lee, JM, Kim, SW, Song, DS, Choi, YK, Kim, JO, Nguyen, HH, Kim, DW, Bahk, YY, Yun, CH & Song, MK 2013, 'Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus', Journal of Microbiology, vol. 51, no. 1, pp. 130-135. https://doi.org/10.1007/s12275-013-2399-z
Shim, Byoung Shik ; Choi, Jung ah ; Song, Ho Hyun ; Park, Sung Moo ; Cheon, In Su ; Jang, Ji Eun ; Woo, Sun Je ; Cho, Chung Hwan ; Song, Min Suk ; Kim, Hyemi ; Song, Kyung Joo ; Lee, Jae Myun ; Kim, Suhng Wook ; Song, Dae Sub ; Choi, Young Ki ; Kim, Jae Ouk ; Nguyen, Huan Huu ; Kim, Dong Wook ; Bahk, Young Yil ; Yun, Cheol Heui ; Song, Man Ki. / Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus. In: Journal of Microbiology. 2013 ; Vol. 51, No. 1. pp. 130-135.
@article{65775fc5824643f792c2de815ac43a5f,
title = "Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus",
abstract = "Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s. l.) route. We found that s. l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i. m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s. l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.",
keywords = "Hemagglutinin, mucosal immune response, non-glycosylation, pandemic, sublingual",
author = "Shim, {Byoung Shik} and Choi, {Jung ah} and Song, {Ho Hyun} and Park, {Sung Moo} and Cheon, {In Su} and Jang, {Ji Eun} and Woo, {Sun Je} and Cho, {Chung Hwan} and Song, {Min Suk} and Hyemi Kim and Song, {Kyung Joo} and Lee, {Jae Myun} and Kim, {Suhng Wook} and Song, {Dae Sub} and Choi, {Young Ki} and Kim, {Jae Ouk} and Nguyen, {Huan Huu} and Kim, {Dong Wook} and Bahk, {Young Yil} and Yun, {Cheol Heui} and Song, {Man Ki}",
year = "2013",
month = "2",
day = "1",
doi = "10.1007/s12275-013-2399-z",
language = "English",
volume = "51",
pages = "130--135",
journal = "Journal of Microbiology",
issn = "1225-8873",
publisher = "Microbiological Society of Korea",
number = "1",

}

TY - JOUR

T1 - Sublingual administration of bacteria-expressed influenza virus hemagglutinin 1 (HA1) induces protection against infection with 2009 pandemic H1N1 influenza virus

AU - Shim, Byoung Shik

AU - Choi, Jung ah

AU - Song, Ho Hyun

AU - Park, Sung Moo

AU - Cheon, In Su

AU - Jang, Ji Eun

AU - Woo, Sun Je

AU - Cho, Chung Hwan

AU - Song, Min Suk

AU - Kim, Hyemi

AU - Song, Kyung Joo

AU - Lee, Jae Myun

AU - Kim, Suhng Wook

AU - Song, Dae Sub

AU - Choi, Young Ki

AU - Kim, Jae Ouk

AU - Nguyen, Huan Huu

AU - Kim, Dong Wook

AU - Bahk, Young Yil

AU - Yun, Cheol Heui

AU - Song, Man Ki

PY - 2013/2/1

Y1 - 2013/2/1

N2 - Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s. l.) route. We found that s. l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i. m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s. l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.

AB - Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s. l.) route. We found that s. l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i. m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s. l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics.

KW - Hemagglutinin

KW - mucosal immune response

KW - non-glycosylation

KW - pandemic

KW - sublingual

UR - http://www.scopus.com/inward/record.url?scp=84874562182&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874562182&partnerID=8YFLogxK

U2 - 10.1007/s12275-013-2399-z

DO - 10.1007/s12275-013-2399-z

M3 - Article

C2 - 23456722

AN - SCOPUS:84874562182

VL - 51

SP - 130

EP - 135

JO - Journal of Microbiology

JF - Journal of Microbiology

SN - 1225-8873

IS - 1

ER -