Abstract
Substance P and neuropeptide Y were discovered as early diagnostic biomarkers of acute myocardial infarction in Korean patients and confirmed using enzyme-linked immunosorbent assay (ELISA). We screened 12 peptides from the sera of Korean acute myocardial infarction (AMI) patients and detected 3 peptides (neuropeptide Y, substance P, and N-terminal pro-B-type natriuretic peptide) to be elevated from patients sera by liquid chromatography mass/mass spectrometry. The elevated concentration of 3 peptides was confirmed by ELISA. The screening results revealed the substance P, neuropeptide Y, and pro-B-type natriuretic peptide (47-76) concentrations were higher in patients sera than in healthy controls. The sensitivity and specificity of substance P for AMI diagnostic marker were 80% and 83%, respectively, and those of neuropeptide Y were 87% and 90%, respectively compared to healthy controls. These results suggest that substance P and neuropeptide Y could be used as early diagnostic biomarkers in patients with AMI.
| Original language | English |
|---|---|
| Pages (from-to) | 158-164 |
| Number of pages | 7 |
| Journal | Bulletin of the Korean Chemical Society |
| Volume | 35 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2014 Jan 20 |
Keywords
- Acute myocardial infarction
- Biomarker
- LC-MS
- Neuropeptide Y
- Substance P
ASJC Scopus subject areas
- Chemistry(all)