Substance P/dexamethasone-encapsulated PLGA scaffold fabricated using supercritical fluid process for calvarial bone regeneration

Su Hee Kim, Ji Eun Kim, Soo Hyun Kim, Youngmee Jung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Poly(lactic-co-glycolic acid) (PLGA) scaffolds encapsulated with substance P (SP) and dexamethasone (Dex) by the supercritical CO2 foaming method were fabricated to treat calvarial bone. We compared the release profiles of SP and Dex according to the incorporation methods using encapsulation or dipping. Ninety percent of the SP or Dex molecules in the scaffolds prepared by the encapsulating method were released by day 14 or day 6, respectively. In vivo real-time assays for human mesenchymal stem cell (hMSC) tracking were performed to confirm the MSC recruitment abilities of the scaffolds. The results showed that the optical intensity of the SP-encapsulated group was 2.59 times higher than that of the phosphate-buffered saline group and 1.3 times higher than that of the SP-dipping group. Furthermore, we compared the angiogenesis activity of the scaffolds. In the SP-encapsulated group, 72.9 ± 2.6% of the vessels showed matured features by 1week, and it increased to 82.0 ± 4.6% after 4weeks. We implanted the scaffolds into rat calvarial defects. After 24weeks, SP- and Dex-encapsulated scaffolds showed 67.1% and 26.2% higher bone formation than those of the Dex-encapsulated group and SP-encapsulated group, respectively, and they formed 36.1% more bone volume compared with the SP- and Dex-dipped scaffolds. Consequently, the results of this study suggest that SP- and Dex-encapsulated scaffolds made by the supercritical CO2 foaming method could be a good treatment modality to treat critical bone defects without cell transplantation by recruiting autologous stem cells and forming new bone tissues.

Original languageEnglish
JournalJournal of Tissue Engineering and Regenerative Medicine
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Bone Regeneration
Supercritical fluids
Substance P
Scaffolds
Dexamethasone
Bone
Acids
Bone and Bones
Stem cells
Defects
polylactic acid-polyglycolic acid copolymer
Milk
Cell Tracking
Encapsulation
Cell Transplantation
Rats
Assays
Phosphates
Mesenchymal Stromal Cells
Osteogenesis

Keywords

  • Bone regeneration
  • Calvarial defect
  • Dexamethasone
  • Stem cell recruitment
  • Substance P
  • Supercritical CO foaming

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

@article{e1173a7d1c2449b1bcd7fc6218b78f2f,
title = "Substance P/dexamethasone-encapsulated PLGA scaffold fabricated using supercritical fluid process for calvarial bone regeneration",
abstract = "Poly(lactic-co-glycolic acid) (PLGA) scaffolds encapsulated with substance P (SP) and dexamethasone (Dex) by the supercritical CO2 foaming method were fabricated to treat calvarial bone. We compared the release profiles of SP and Dex according to the incorporation methods using encapsulation or dipping. Ninety percent of the SP or Dex molecules in the scaffolds prepared by the encapsulating method were released by day 14 or day 6, respectively. In vivo real-time assays for human mesenchymal stem cell (hMSC) tracking were performed to confirm the MSC recruitment abilities of the scaffolds. The results showed that the optical intensity of the SP-encapsulated group was 2.59 times higher than that of the phosphate-buffered saline group and 1.3 times higher than that of the SP-dipping group. Furthermore, we compared the angiogenesis activity of the scaffolds. In the SP-encapsulated group, 72.9 ± 2.6{\%} of the vessels showed matured features by 1week, and it increased to 82.0 ± 4.6{\%} after 4weeks. We implanted the scaffolds into rat calvarial defects. After 24weeks, SP- and Dex-encapsulated scaffolds showed 67.1{\%} and 26.2{\%} higher bone formation than those of the Dex-encapsulated group and SP-encapsulated group, respectively, and they formed 36.1{\%} more bone volume compared with the SP- and Dex-dipped scaffolds. Consequently, the results of this study suggest that SP- and Dex-encapsulated scaffolds made by the supercritical CO2 foaming method could be a good treatment modality to treat critical bone defects without cell transplantation by recruiting autologous stem cells and forming new bone tissues.",
keywords = "Bone regeneration, Calvarial defect, Dexamethasone, Stem cell recruitment, Substance P, Supercritical CO foaming",
author = "Kim, {Su Hee} and Kim, {Ji Eun} and Kim, {Soo Hyun} and Youngmee Jung",
year = "2017",
doi = "10.1002/term.2260",
language = "English",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Ltd",

}

TY - JOUR

T1 - Substance P/dexamethasone-encapsulated PLGA scaffold fabricated using supercritical fluid process for calvarial bone regeneration

AU - Kim, Su Hee

AU - Kim, Ji Eun

AU - Kim, Soo Hyun

AU - Jung, Youngmee

PY - 2017

Y1 - 2017

N2 - Poly(lactic-co-glycolic acid) (PLGA) scaffolds encapsulated with substance P (SP) and dexamethasone (Dex) by the supercritical CO2 foaming method were fabricated to treat calvarial bone. We compared the release profiles of SP and Dex according to the incorporation methods using encapsulation or dipping. Ninety percent of the SP or Dex molecules in the scaffolds prepared by the encapsulating method were released by day 14 or day 6, respectively. In vivo real-time assays for human mesenchymal stem cell (hMSC) tracking were performed to confirm the MSC recruitment abilities of the scaffolds. The results showed that the optical intensity of the SP-encapsulated group was 2.59 times higher than that of the phosphate-buffered saline group and 1.3 times higher than that of the SP-dipping group. Furthermore, we compared the angiogenesis activity of the scaffolds. In the SP-encapsulated group, 72.9 ± 2.6% of the vessels showed matured features by 1week, and it increased to 82.0 ± 4.6% after 4weeks. We implanted the scaffolds into rat calvarial defects. After 24weeks, SP- and Dex-encapsulated scaffolds showed 67.1% and 26.2% higher bone formation than those of the Dex-encapsulated group and SP-encapsulated group, respectively, and they formed 36.1% more bone volume compared with the SP- and Dex-dipped scaffolds. Consequently, the results of this study suggest that SP- and Dex-encapsulated scaffolds made by the supercritical CO2 foaming method could be a good treatment modality to treat critical bone defects without cell transplantation by recruiting autologous stem cells and forming new bone tissues.

AB - Poly(lactic-co-glycolic acid) (PLGA) scaffolds encapsulated with substance P (SP) and dexamethasone (Dex) by the supercritical CO2 foaming method were fabricated to treat calvarial bone. We compared the release profiles of SP and Dex according to the incorporation methods using encapsulation or dipping. Ninety percent of the SP or Dex molecules in the scaffolds prepared by the encapsulating method were released by day 14 or day 6, respectively. In vivo real-time assays for human mesenchymal stem cell (hMSC) tracking were performed to confirm the MSC recruitment abilities of the scaffolds. The results showed that the optical intensity of the SP-encapsulated group was 2.59 times higher than that of the phosphate-buffered saline group and 1.3 times higher than that of the SP-dipping group. Furthermore, we compared the angiogenesis activity of the scaffolds. In the SP-encapsulated group, 72.9 ± 2.6% of the vessels showed matured features by 1week, and it increased to 82.0 ± 4.6% after 4weeks. We implanted the scaffolds into rat calvarial defects. After 24weeks, SP- and Dex-encapsulated scaffolds showed 67.1% and 26.2% higher bone formation than those of the Dex-encapsulated group and SP-encapsulated group, respectively, and they formed 36.1% more bone volume compared with the SP- and Dex-dipped scaffolds. Consequently, the results of this study suggest that SP- and Dex-encapsulated scaffolds made by the supercritical CO2 foaming method could be a good treatment modality to treat critical bone defects without cell transplantation by recruiting autologous stem cells and forming new bone tissues.

KW - Bone regeneration

KW - Calvarial defect

KW - Dexamethasone

KW - Stem cell recruitment

KW - Substance P

KW - Supercritical CO foaming

UR - http://www.scopus.com/inward/record.url?scp=85019993333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019993333&partnerID=8YFLogxK

U2 - 10.1002/term.2260

DO - 10.1002/term.2260

M3 - Article

C2 - 28568973

AN - SCOPUS:85019993333

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

ER -